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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 7 — Jul. 1, 2013
  • pp: 1204–1213

Multiphoton morpho-functional imaging of healthy colon mucosa, adenomatous polyp and adenocarcinoma

Riccardo Cicchi, Alessandro Sturiale, Gabriella Nesi, Dimitrios Kapsokalyvas, Giovanni Alemanno, Francesco Tonelli, and Francesco S. Pavone  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 7, pp. 1204-1213 (2013)

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Two-photon spectral resolved imaging was used to image fresh human biopsies of colon tissue and to characterize healthy colon mucosa, adenomatous polyp and adenocarcinoma by means of a morpho-functional analysis. Morphological examination, performed using endogenous tissue fluorescence, discriminated adenomatous and adenocarcinoma tissues from normal mucosa in terms of cellular asymmetry and nucleus-to-cytoplasm ratio. Good agreement was found between multiphoton images and histological examination performed on the same samples. Further characterization, performed by means of spectral-resolved analysis of NADH and FAD fluorescence, demonstrated an altered metabolic activity in both adenomatous and adenocarcinoma tissues compared to healthy mucosa. This morpho-functional approach may represent a powerful method to be used in combination with endoscopy for in vivo optical diagnosis of colon cancer and may be extended to other tissues.

© 2013 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Functional Imaging

Original Manuscript: April 16, 2013
Revised Manuscript: May 9, 2013
Manuscript Accepted: May 9, 2013
Published: June 24, 2013

Riccardo Cicchi, Alessandro Sturiale, Gabriella Nesi, Dimitrios Kapsokalyvas, Giovanni Alemanno, Francesco Tonelli, and Francesco S. Pavone, "Multiphoton morpho-functional imaging of healthy colon mucosa, adenomatous polyp and adenocarcinoma," Biomed. Opt. Express 4, 1204-1213 (2013)

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