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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 8 — Aug. 1, 2013
  • pp: 1236–1254

Ultrahigh speed spectral-domain optical coherence microscopy

Hsiang-Chieh Lee, Jonathan J. Liu, Yuri Sheikine, Aaron D. Aguirre, James L. Connolly, and James G. Fujimoto  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 8, pp. 1236-1254 (2013)

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We demonstrate a compact, ultrahigh speed spectral-domain optical coherence microscopy (SD-OCM) system for multiscale imaging of specimens at 840 nm. Using a high speed 512-pixel line scan camera, an imaging speed of 210,000 A-scans per second was demonstrated. Interchangeable water immersion objectives with magnifications of 10×, 20×, and 40× provided co-registered en face cellular-resolution imaging over several size scales. Volumetric OCM data sets and en face OCM images were demonstrated on both normal and pathological human colon and kidney specimens ex vivo with an axial resolution of ~4.2 µm, and transverse resolutions of ~2.9 µm (10×), ~1.7 µm (20×), and ~1.1 µm (40×) in tissue. In addition, en face OCM images acquired with high numerical aperture over an extended field-of-view (FOV) were demonstrated using image mosaicking. Comparison between en face OCM images among different transverse and axial resolutions was demonstrated, which promises to help the design and evaluation of imaging performance of Fourier domain OCM systems at different resolution regimes.

© 2013 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.1790) Microscopy : Confocal microscopy

ToC Category:
Optical Coherence Tomography

Original Manuscript: April 16, 2013
Revised Manuscript: June 12, 2013
Manuscript Accepted: June 12, 2013
Published: July 1, 2013

Hsiang-Chieh Lee, Jonathan J. Liu, Yuri Sheikine, Aaron D. Aguirre, James L. Connolly, and James G. Fujimoto, "Ultrahigh speed spectral-domain optical coherence microscopy," Biomed. Opt. Express 4, 1236-1254 (2013)

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