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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 8 — Aug. 1, 2013
  • pp: 1380–1389

Simultaneous three-dimensional photoacoustic and laser-ultrasound tomography

Gerhild Wurzinger, Robert Nuster, Nicole Schmitner, Sibylle Gratt, Dirk Meyer, and Günther Paltauf  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 8, pp. 1380-1389 (2013)

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A tomographic setup that provides the co-registration of photoacoustic (PA) and ultrasound (US) images is presented. For pulse-echo US-tomography laser-induced broadband plane ultrasonic waves are produced by illuminating an optically absorbing target with a short near-infrared laser pulse. Part of the same pulse is frequency doubled and used for the generation of PA waves within the object of interest. The laser-generated plane waves are scattered at the imaging object and measured with the same interferometric detector that also acquires the photoacoustic signals. After collection and separation of the data image reconstruction is done using back-projection resulting in three-dimensional, co-registered PA and US images. The setup is characterized and the resolution in PA and US mode is estimated to be about 85 µm and 40 µm, respectively. Besides measurements on phantoms the performance is also tested on a biological sample.

© 2013 Optical Society of America

OCIS Codes
(110.5120) Imaging systems : Photoacoustic imaging
(110.7170) Imaging systems : Ultrasound

ToC Category:
Photoacoustic Imaging and Spectroscopy

Original Manuscript: April 16, 2013
Revised Manuscript: June 27, 2013
Manuscript Accepted: July 17, 2013
Published: July 19, 2013

Gerhild Wurzinger, Robert Nuster, Nicole Schmitner, Sibylle Gratt, Dirk Meyer, and Günther Paltauf, "Simultaneous three-dimensional photoacoustic and laser-ultrasound tomography," Biomed. Opt. Express 4, 1380-1389 (2013)

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  1. L. V. Wang, S. Hu, “Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs,” Science 335(6075), 1458–1462 (2012). [CrossRef] [PubMed]
  2. J. Laufer, P. Johnson, E. Zhang, B. Treeby, B. Cox, B. Pedley, P. Beard, “In vivo preclinical photoacoustic imaging of tumor vasculature development and therapy,” J. Biomed. Opt. 17(5), 056016 (2012). [CrossRef] [PubMed]
  3. M. H. Xu, L. V. Wang, “Photoacoustic Imaging in Biomedicine,” Rev. Sci. Instrum. 77(4), 041101 (2006). [CrossRef]
  4. P. Beard, “Biomedical photoacoustic imaging,” Interface Focus 1(4), 602–631 (2011). [CrossRef] [PubMed]
  5. E. Z. Zhang, J. G. Laufer, R. B. Pedley, P. C. Beard, “In vivo high-resolution 3D photoacoustic imaging of superficial vascular anatomy,” Phys. Med. Biol. 54(4), 1035–1046 (2009). [CrossRef] [PubMed]
  6. J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, M. Frenz, “Combined Ultrasound and Optoacoustic System for Real-Time High-Contrast Vascular Imaging in Vivo,” IEEE Trans. Med. Imaging 24(4), 436–440 (2005). [CrossRef] [PubMed]
  7. R. G. M. Kolkman, P. J. Brands, W. Steenbergen, T. G. van Leeuwen, “Real-time in vivo photoacoustic and ultrasound imaging,” J. Biomed. Opt. 13(5), 050510 (2008). [CrossRef] [PubMed]
  8. S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. G. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, A. Oraevsky, “Combined ultrasound, optoacoustic and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing 5320, 101–112 (2004).
  9. T. Harrison, J. C. Ranasinghesagara, H. Lu, K. Mathewson, A. Walsh, R. J. Zemp, “Combined photoacoustic and ultrasound biomicroscopy,” Opt. Express 17(24), 22041–22046 (2009). [CrossRef] [PubMed]
  10. M. Jaeger, D. Harris-Birtill, A. Gertsch, E. O’Flynn, J. Bamber, “Deformation-compensated averaging for clutter reduction in epiphotoacoustic imaging in vivo,” J. Biomed. Opt. 17(6), 066007 (2012). [CrossRef] [PubMed]
  11. P. C. Beard, E. Z. Zhang, B. T. Cox, “Transparent Fabry Perot polymer film ultrasound array for backward-mode photoacoustic imaging,” Photons Plus Ultrasound: Imaging and Sensing 5320, 230–237 (2004).
  12. G. Paltauf, R. Nuster, M. Haltmeier, P. Burgholzer, “Photoacoustic tomography using a Mach-Zehnder interferometer as an acoustic line detector,” Appl. Opt. 46(16), 3352–3358 (2007). [CrossRef] [PubMed]
  13. M. Jaeger, J. J. Niederhauser, M. Hejazi, M. Frenz, “Diffraction-free acoustic detection for optoacoustic depth profiling of tissue using an optically transparent polyvinylidene fluoride pressure transducer operated in backward and forward mode,” J. Biomed. Opt. 10(2), 024035 (2005). [CrossRef] [PubMed]
  14. R. Nuster, S. Gratt, K. Passler, H. Grun, T. Berer, P. Burgholzer, G. Paltauf, “Comparison of optical and piezoelectric integrating line detectors,” Photons Plus Ultrasound: Imaging and Sensing 2009, 7177 (2009).
  15. R. Nuster, N. Schmitner, G. Wurzinger, S. Gratt, W. Salvenmoser, D. Meyer, G. Paltauf, “Hybrid photoacoustic and ultrasound section imaging with optical ultrasound detection,” J Biophotonics 6(6-7), 549–559 (2013). [CrossRef] [PubMed]
  16. S. Manohar, R. G. H. Willemink, F. van der Heijden, C. H. Slump, T. G. van Leeuwen, “Concomitant speed-of-sound tomography in photoacoustic imaging,” Appl. Phys. Lett. 91(13), 131911 (2007). [CrossRef]
  17. J. Jose, R. G. H. Willemink, S. Resink, D. Piras, J. C. G. van Hespen, C. H. Slump, W. Steenbergen, T. G. van Leeuwen, S. Manohar, “Passive element enriched photoacoustic computed tomography (PER PACT) for simultaneous imaging of acoustic propagation properties and light absorption,” Opt. Express 19(3), 2093–2104 (2011). [CrossRef] [PubMed]
  18. S. Resink, J. Jose, R. G. H. Willemink, C. H. Slump, W. Steenbergen, T. G. van Leeuwen, S. Manohar, “Multiple passive element enriched photoacoustic computed tomography,” Opt. Lett. 36(15), 2809–2811 (2011). [CrossRef] [PubMed]
  19. S. A. Ermilov, A. Conjusteau, T. Hernandez, R. Su, V. Nadvoretskiy, D. Tsyboulski, F. Anis, M. A. Anastasio, and A. A. Oraevsky, “3D laser optoacoustic ultrasonic imaging system for preclinical research,” 85810N–85810N (2013).
  20. X. Jin, L. V. Wang, “Thermoacoustic tomography with correction for acoustic speed variations,” Phys. Med. Biol. 51(24), 6437–6448 (2006). [CrossRef] [PubMed]
  21. H. B. Jiang, Z. Yuan, X. J. Gu, “Spatially Varying Optical and Acoustic Property Reconstruction Using Finite-Element-Based Photoacoustic Tomography,” J. Opt. Soc. Am. A 23(4), 878–888 (2006). [CrossRef] [PubMed]
  22. R. Nuster, M. Holotta, C. Kremser, H. Grossauer, P. Burgholzer, G. Paltauf, “Photoacoustic microtomography using optical interferometric detection,” J. Biomed. Opt. 15(2), 021307 (2010). [CrossRef] [PubMed]
  23. M. H. Xu, L. V. Wang, “Universal back-projection algorithm for photoacoustic computed tomography,” Phys. Rev. E Stat. Nonlin. Soft Matter Phys. 71(1), 016706 (2005). [CrossRef] [PubMed]
  24. G. Paltauf, R. Nuster, M. Haltmeier, P. Burgholzer, “Experimental evaluation of reconstruction algorithms for limited view photoacoustic tomography with line detectors,” Inverse Probl. 23(6), S81–S94 (2007). [CrossRef]
  25. G. Paltauf, R. Nuster, P. Burgholzer, “Weight factors for limited angle photoacoustic tomography,” Phys. Med. Biol. 54(11), 3303–3314 (2009). [CrossRef] [PubMed]
  26. G. Paltauf, R. Nuster, P. Burgholzer, “Characterization of integrating ultrasound detectors for photoacoustic tomography,” J. Appl. Phys. 105(10), 102026 (2009). [CrossRef]
  27. H. R. Shive, “Zebrafish models for human cancer,” Vet. Pathol. 50(3), 468–482 (2013). [CrossRef] [PubMed]
  28. W. Choi, K. D. Poss, “Cardiac Regeneration,” Curr. Top. Dev. Biol. 100, 319 (2012).
  29. C. Kizil, J. Kaslin, V. Kroehne, M. Brand, “Adult neurogenesis and brain regeneration in zebrafish,” Dev. Neurobiol. 72(3), 429–461 (2012). [CrossRef] [PubMed]
  30. J. B. Moss, P. Koustubhan, M. Greenman, M. J. Parsons, I. Walter, L. G. Moss, “Regeneration of the Pancreas in Adult Zebrafish,” Diabetes 58(8), 1844–1851 (2009). [CrossRef] [PubMed]
  31. S. Liu, S. D. Leach, “Screening Pancreatic Oncogenes in Zebrafish Using the Gal4/UAS System,” Methods Cell Biol. 105, 367–381 (2011). [CrossRef] [PubMed]
  32. Z. Cao, L. D. Jensen, P. Rouhi, K. Hosaka, T. Länne, J. F. Steffensen, E. Wahlberg, Y. Cao, “Hypoxia-induced retinopathy model in adult zebrafish,” Nat. Protoc. 5(12), 1903–1910 (2010). [CrossRef] [PubMed]
  33. R. Ma, M. Distel, X. L. Deán-Ben, V. Ntziachristos, D. Razansky, “Non-invasive whole-body imaging of adult zebrafish with optoacoustic tomography,” Phys. Med. Biol. 57(22), 7227–7237 (2012). [CrossRef] [PubMed]
  34. S. Ye, R. Yang, J. Xiong, K. K. Shung, Q. Zhou, C. Li, Q. Ren, “Label-free imaging of zebrafish larvae in vivo by photoacoustic microscopy,” Biomed. Opt. Express 3(2), 360–365 (2012). [CrossRef] [PubMed]
  35. W. Goessling, T. E. North, L. I. Zon, “Ultrasound biomicroscopy permits in vivo characterization of zebrafish liver tumors,” Nat. Methods 4(7), 551–553 (2007). [CrossRef] [PubMed]
  36. L. Sun, C. L. Lien, X. Xu, K. K. Shung, “In vivo cardiac imaging of adult zebrafish using high frequency ultrasound (45-75 MHz),” Ultrasound Med. Biol. 34(1), 31–39 (2008). [CrossRef] [PubMed]
  37. Y. Xu, L. V. Wang, G. Ambartsoumian, P. Kuchment, “Reconstructions in limited-view thermoacoustic tomography,” Med. Phys. 31(4), 724–733 (2004). [CrossRef] [PubMed]
  38. X. L. Deán-Ben, V. Ntziachristos, D. Razansky, “Artefact reduction in optoacoustic tomographic imaging by estimating the distribution of acoustic scatterers,” J. Biomed. Opt. 17(11), 110504 (2012). [CrossRef] [PubMed]

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