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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 8 — Aug. 1, 2013
  • pp: 1451–1463

Continuous real-time photoacoustic demodulation via field programmable gate array for dynamic imaging of zebrafish cardiac cycle

Scott P. Mattison, Ryan L. Shelton, Ryan T. Maxson, and Brian E. Applegate  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 8, pp. 1451-1463 (2013)
http://dx.doi.org/10.1364/BOE.4.001451


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Abstract

A four dimensional data set of the cardiac cycle of a zebrafish embryo was acquired using postacquisition synchronization of real time photoacoustic b-scans. Utilizing an off-axis photoacoustic microscopy (OA-PAM) setup, we have expanded upon our previous work with OA-PAM to develop a system that can sustain 100 kHz line rates while demodulating the bipolar photoacoustic signal in real-time. Real-time processing was accomplished by quadrature demodulation on a Field Programmable Gate Array (FPGA) in line with the signal digitizer. Simulated data acquisition verified the system is capable of real-time processing up to a line rate of 1 MHz. Galvanometer-scanning of the excitation laser inside the focus of the ultrasonic transducer enables real data acquisition of a 200 by 200 by 200 pixel, volumetric data set across a 2 millimeter field of view at a rate of 2.5 Hz.

© 2013 OSA

OCIS Codes
(100.2000) Image processing : Digital image processing
(110.5120) Imaging systems : Photoacoustic imaging
(110.5125) Imaging systems : Photoacoustics

ToC Category:
Photoacoustic Imaging and Spectroscopy

History
Original Manuscript: June 10, 2013
Revised Manuscript: July 22, 2013
Manuscript Accepted: July 23, 2013
Published: July 29, 2013

Citation
Scott P. Mattison, Ryan L. Shelton, Ryan T. Maxson, and Brian E. Applegate, "Continuous real-time photoacoustic demodulation via field programmable gate array for dynamic imaging of zebrafish cardiac cycle," Biomed. Opt. Express 4, 1451-1463 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-8-1451


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