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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 2 — Feb. 1, 2014
  • pp: 645–652

Optical axial scanning in confocal microscopy using an electrically tunable lens

Joey M. Jabbour, Bilal H. Malik, Cory Olsovsky, Rodrigo Cuenca, Shuna Cheng, Javier A. Jo, Yi-Shing Lisa Cheng, John M. Wright, and Kristen C. Maitland  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 2, pp. 645-652 (2014)
http://dx.doi.org/10.1364/BOE.5.000645


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Abstract

This paper presents the use and characterization of an electrically focus tunable lens to perform axial scanning in a confocal microscope. Lateral and axial resolution are characterized over a >250 µm axial scan range. Confocal microscopy using optical axial scanning is demonstrated in epithelial tissue and compared to traditional stage scanning. By enabling rapid axial scanning, minimizing motion artifacts, and reducing mechanical complexity, this technique has potential to enhance in vivo three-dimensional imaging in confocal endomicroscopy.

© 2014 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: November 25, 2013
Revised Manuscript: January 28, 2014
Manuscript Accepted: January 28, 2014
Published: January 30, 2014

Citation
Joey M. Jabbour, Bilal H. Malik, Cory Olsovsky, Rodrigo Cuenca, Shuna Cheng, Javier A. Jo, Yi-Shing Lisa Cheng, John M. Wright, and Kristen C. Maitland, "Optical axial scanning in confocal microscopy using an electrically tunable lens," Biomed. Opt. Express 5, 645-652 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-2-645


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References

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