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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 3 — Mar. 1, 2014
  • pp: 690–700

4D tracking of clinical seminal samples for quantitative characterization of motility parameters

Giuseppe Di Caprio, Ahmed El Mallahi, Pietro Ferraro, Roberta Dale, Gianfranco Coppola, Brian Dale, Giuseppe Coppola, and Frank Dubois  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 3, pp. 690-700 (2014)
http://dx.doi.org/10.1364/BOE.5.000690


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Abstract

In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm.

© 2014 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(180.0180) Microscopy : Microscopy
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: October 14, 2013
Revised Manuscript: December 6, 2013
Manuscript Accepted: December 30, 2013
Published: February 11, 2014

Citation
Giuseppe Di Caprio, Ahmed El Mallahi, Pietro Ferraro, Roberta Dale, Gianfranco Coppola, Brian Dale, Giuseppe Coppola, and Frank Dubois, "4D tracking of clinical seminal samples for quantitative characterization of motility parameters," Biomed. Opt. Express 5, 690-700 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-3-690


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