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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 4 — Apr. 1, 2014
  • pp: 1099–1113

Motion-artifact-robust, polarization-resolved second-harmonic-generation microscopy based on rapid polarization switching with electro-optic Pockells cell and its application to in vivo visualization of collagen fiber orientation in human facial skin

Yuji Tanaka, Eiji Hase, Shuichiro Fukushima, Yuki Ogura, Toyonobu Yamashita, Tetsuji Hirao, Tsutomu Araki, and Takeshi Yasui  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 4, pp. 1099-1113 (2014)
http://dx.doi.org/10.1364/BOE.5.001099


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Abstract

Polarization-resolved second-harmonic-generation (PR-SHG) microscopy is a powerful tool for investigating collagen fiber orientation quantitatively with low invasiveness. However, the waiting time for the mechanical polarization rotation makes it too sensitive to motion artifacts and hence has hampered its use in various applications in vivo. In the work described in this article, we constructed a motion-artifact-robust, PR-SHG microscope based on rapid polarization switching at every pixel with an electro-optic Pockells cell (PC) in synchronization with step-wise raster scanning of the focus spot and alternate data acquisition of a vertical-polarization-resolved SHG signal and a horizontal-polarization-resolved one. The constructed PC-based PR-SHG microscope enabled us to visualize orientation mapping of dermal collagen fiber in human facial skin in vivo without the influence of motion artifacts. Furthermore, it implied the location and/or age dependence of the collagen fiber orientation in human facial skin. The robustness to motion artifacts in the collagen orientation measurement will expand the application scope of SHG microscopy in dermatology and collagen-related fields.

© 2014 Optical Society of America

OCIS Codes
(170.1870) Medical optics and biotechnology : Dermatology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.4160) Nonlinear optics : Multiharmonic generation
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: January 13, 2014
Revised Manuscript: February 16, 2014
Manuscript Accepted: March 2, 2014
Published: March 7, 2014

Citation
Yuji Tanaka, Eiji Hase, Shuichiro Fukushima, Yuki Ogura, Toyonobu Yamashita, Tetsuji Hirao, Tsutomu Araki, and Takeshi Yasui, "Motion-artifact-robust, polarization-resolved second-harmonic-generation microscopy based on rapid polarization switching with electro-optic Pockells cell and its application to in vivo visualization of collagen fiber orientation in human facial skin," Biomed. Opt. Express 5, 1099-1113 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-4-1099


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