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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 5 — May. 1, 2014
  • pp: 1483–1493

Real-time high resolution laser speckle imaging of cerebral vascular changes in a rodent photothrombosis model

Qi Liu, Yao Li, Hongyang Lu, and Shanbao Tong  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 5, pp. 1483-1493 (2014)
http://dx.doi.org/10.1364/BOE.5.001483


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Abstract

The study of hemodynamic and vascular changes following ischemic stroke is of great importance in the understanding of physiological and pathological processes during the thrombus formation. The photothrombosis model is preferred by researchers in stroke study for its minimal invasiveness, controllable infarct volume and lesion location. Nevertheless, there is a lack in high spatiotemporal resolution techniques for real time monitoring of cerebral blood flow (CBF) changes in 2D-profile. In this study, we implemented a microscopic laser speckle imaging (LSI) system to detect CBF and other vascular changes in the rodent model of photothrombotic stroke. Using a high resolution and high speed CCD (640 × 480 pixels, 60 fps), online image registration technique, and automatic parabolic curve fitting, we obtained real time CBF and blood velocity profile (BVP) changes in cortical vessels. Real time CBF and BVP monitoring has been shown to reveal details of vascular disturbances and the stages of blood coagulation in photothrombotic stroke. Moreover, LSI also provides information on additional parameters including vessel morphologic size, blood flow centerline velocity and CBF spatiotemporal fluctuations, which are very important for understanding the physiology and neurovascular pathology in the photothrombosis model.

© 2014 Optical Society of America

OCIS Codes
(110.6150) Imaging systems : Speckle imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Speckle Imaging and Diagnostics

History
Original Manuscript: December 5, 2013
Revised Manuscript: March 16, 2014
Manuscript Accepted: April 8, 2014
Published: April 11, 2014

Citation
Qi Liu, Yao Li, Hongyang Lu, and Shanbao Tong, "Real-time high resolution laser speckle imaging of cerebral vascular changes in a rodent photothrombosis model," Biomed. Opt. Express 5, 1483-1493 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-5-1483


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