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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 5 — May. 1, 2014
  • pp: 1677–1689

Intravital imaging of mouse colonic adenoma using MMP-based molecular probes with multi-channel fluorescence endoscopy

Gyungseok Oh, Su Woong Yoo, Yebin Jung, Yeon-Mi Ryu, Youngrong Park, Sang-Yeob Kim, Ki Hean Kim, Sungjee Kim, Seung-Jae Myung, and Euiheon Chung  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 5, pp. 1677-1689 (2014)
http://dx.doi.org/10.1364/BOE.5.001677


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Abstract

Intravital imaging has provided molecular, cellular and anatomical insight into the study of tumor. Early detection and treatment of gastrointestinal (GI) diseases can be enhanced with specific molecular markers and endoscopic imaging modalities. We present a wide-field multi-channel fluorescence endoscope to screen GI tract for colon cancer using multiple molecular probes targeting matrix metalloproteinases (MMP) conjugated with quantum dots (QD) in AOM/DSS mouse model. MMP9 and MMP14 antibody (Ab)-QD conjugates demonstrate specific binding to colonic adenoma. The average target-to-background (T/B) ratios are 2.10 ± 0.28 and 1.78 ± 0.18 for MMP14 Ab-QD and MMP9 Ab-QD, respectively. The overlap between the two molecular probes is 67.7 ± 8.4%. The presence of false negative indicates that even more number of targeting could increase the sensitivity of overall detection given heterogeneous molecular expression in tumors. Our approach indicates potential for the screening of small or flat lesions that are precancerous.

© 2014 Optical Society of America

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

ToC Category:
Optics in Cancer Research

History
Original Manuscript: March 14, 2014
Revised Manuscript: April 19, 2014
Manuscript Accepted: April 21, 2014
Published: April 29, 2014

Citation
Gyungseok Oh, Su Woong Yoo, Yebin Jung, Yeon-Mi Ryu, Youngrong Park, Sang-Yeob Kim, Ki Hean Kim, Sungjee Kim, Seung-Jae Myung, and Euiheon Chung, "Intravital imaging of mouse colonic adenoma using MMP-based molecular probes with multi-channel fluorescence endoscopy," Biomed. Opt. Express 5, 1677-1689 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-5-1677


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