OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 6 — Jun. 1, 2014
  • pp: 1721–1730

Off-axis digital holographic camera for quantitative phase microscopy

Zahra Monemhaghdoust, Frédéric Montfort, Yves Emery, Christian Depeursinge, and Christophe Moser  »View Author Affiliations


Biomedical Optics Express, Vol. 5, Issue 6, pp. 1721-1730 (2014)
http://dx.doi.org/10.1364/BOE.5.001721


View Full Text Article

Enhanced HTML    Acrobat PDF (1226 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a single shot. A thick holographic grating filters the beam containing the sample information in two dimensions through diffraction. The filtered beam creates the reference arm of the interferometer. The spatial filtering method, based on the high angular selectivity of the thick grating, reduces the alignment sensitivity to angular displacements compared with pinhole based Fourier filtering. The addition of a thin holographic grating alters the coherence plane tilt introduced by the thick grating so as to create high-visibility interference over the entire field of view. The acquired full-field off-axis holograms are processed to retrieve the amplitude and phase information of the sample. The system produces phase images of cheek cells qualitatively similar to phase images extracted with a standard commercial DHM.

© 2014 Optical Society of America

OCIS Codes
(050.1950) Diffraction and gratings : Diffraction gratings
(050.7330) Diffraction and gratings : Volume gratings
(110.0180) Imaging systems : Microscopy
(180.3170) Microscopy : Interference microscopy
(330.6110) Vision, color, and visual optics : Spatial filtering
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: February 26, 2014
Revised Manuscript: April 24, 2014
Manuscript Accepted: April 27, 2014
Published: May 1, 2014

Citation
Zahra Monemhaghdoust, Frédéric Montfort, Yves Emery, Christian Depeursinge, and Christophe Moser, "Off-axis digital holographic camera for quantitative phase microscopy," Biomed. Opt. Express 5, 1721-1730 (2014)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-5-6-1721


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. E. Cuche, P. Marquet, and C. Depeursinge, “Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms,” Appl. Opt.38(34), 6994–7001 (1999). [CrossRef] [PubMed]
  2. E. Cuche, F. Bevilacqua, and C. Depeursinge, “Digital holography for quantitative phase-contrast imaging,” Opt. Lett.24(5), 291–293 (1999). [CrossRef] [PubMed]
  3. P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, “Digital holographic microscopy: A noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett.30(5), 468–470 (2005). [CrossRef] [PubMed]
  4. H. Ding and G. Popescu, “Instantaneous spatial light interference microscopy,” Opt. Express18(2), 1569–1575 (2010). [CrossRef] [PubMed]
  5. Z. Monemhaghdoust, F. Montfort, Y. Emery, C. Depeursinge, and C. Moser, “Dual wavelength full field imaging in low coherence digital holographic microscopy,” Opt. Express19(24), 24005–24022 (2011). [CrossRef] [PubMed]
  6. Z. Monemahghdoust, F. Montfort, E. Cuche, Y. Emery, C. Depeursinge, and C. Moser, “Full field vertical scanning in short coherence digital holographic microscope,” Opt. Express21(10), 12643–12650 (2013). [CrossRef] [PubMed]
  7. T. Ikeda, G. Popescu, R. R. Dasari, and M. S. Feld, “Hilbert phase microscopy for investigating fast dynamics in transparent systems,” Opt. Lett.30(10), 1165–1167 (2005). [CrossRef] [PubMed]
  8. P. Girshovitz and N. T. Shaked, “Compact and portable low-coherence interferometer with off-axis geometry for quantitative phase microscopy and nanoscopy,” Opt. Express21(5), 5701–5714 (2013). [CrossRef] [PubMed]
  9. B. Bhaduri, H. Pham, M. Mir, and G. Popescu, “Diffraction phase microscopy with white light,” Opt. Lett.37(6), 1094–1096 (2012). [CrossRef] [PubMed]
  10. J. E. Ludman, J. R. Riccobono, N. O. Reinhand, I. V. Semenova, Y. L. Korzinin, and M. S. Shahriar, “Holographic nonspatial filter,” Proc. SPIE2532, 481–490 (1995). [CrossRef]
  11. J. E. Ludman, J. R. Riccobono, N. O. Reinhand, I. V. Semenova, Y. L. Korzinin, and M. S. Shahriar, “Nonspatial filter for laser beams,” Quantum Electron.26(12), 1093–1096 (1996). [CrossRef]
  12. O. M. Efimov, L. B. Glebov, L. N. Glebova, K. C. Richardson, and V. I. Smirnov, “High-efficiency bragg gratings in photothermorefractive glass,” Appl. Opt.38(4), 619–627 (1999). [CrossRef] [PubMed]
  13. I. V. Ciapurin, L. B. Glebov, and V. I. Smirnov, “Modeling of Gaussian beam diffraction on volume bragg gratings in PTR glass,” Proc. SPIE5742, 183–194 (2005). [CrossRef]
  14. I. V. Ciapurin, V. I. Smirnov, and L. B. Glebov, “Modeling of phase volume diffractive gratings, part1: transmitting sinusoidal uniform gratings,” Opt. Eng.45(1), 015802 (2006). [CrossRef]
  15. H. Kogelnik, “Coupled wave theory for thick hologram gratings,” Bell Syst. Tech. J.48(9), 2909–2947 (1969). [CrossRef]
  16. M. Henrion, J. E. Ludman, G. Sobolev, M. S. Shahriar, S. Soboleva, and P. Hemmer, “Two-dimensional holographic nonspatial filtering for laser beams,” Proc. SPIE3417, 195–206 (1998). [CrossRef]
  17. J. E. Ludman, T. D. Upton, and D. Coolidge, “Single element holographic non spatial filter,” Proc. SPIE5005, 375–379 (2003). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited