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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 6 — Jun. 1, 2014
  • pp: 1721–1730

Off-axis digital holographic camera for quantitative phase microscopy

Zahra Monemhaghdoust, Frédéric Montfort, Yves Emery, Christian Depeursinge, and Christophe Moser  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 6, pp. 1721-1730 (2014)

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We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a single shot. A thick holographic grating filters the beam containing the sample information in two dimensions through diffraction. The filtered beam creates the reference arm of the interferometer. The spatial filtering method, based on the high angular selectivity of the thick grating, reduces the alignment sensitivity to angular displacements compared with pinhole based Fourier filtering. The addition of a thin holographic grating alters the coherence plane tilt introduced by the thick grating so as to create high-visibility interference over the entire field of view. The acquired full-field off-axis holograms are processed to retrieve the amplitude and phase information of the sample. The system produces phase images of cheek cells qualitatively similar to phase images extracted with a standard commercial DHM.

© 2014 Optical Society of America

OCIS Codes
(050.1950) Diffraction and gratings : Diffraction gratings
(050.7330) Diffraction and gratings : Volume gratings
(110.0180) Imaging systems : Microscopy
(180.3170) Microscopy : Interference microscopy
(330.6110) Vision, color, and visual optics : Spatial filtering
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: February 26, 2014
Revised Manuscript: April 24, 2014
Manuscript Accepted: April 27, 2014
Published: May 1, 2014

Zahra Monemhaghdoust, Frédéric Montfort, Yves Emery, Christian Depeursinge, and Christophe Moser, "Off-axis digital holographic camera for quantitative phase microscopy," Biomed. Opt. Express 5, 1721-1730 (2014)

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