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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 7 — Jul. 1, 2014
  • pp: 2113–2124

Optical coherence micro-elastography: mechanical-contrast imaging of tissue microstructure

Brendan F. Kennedy, Robert A. McLaughlin, Kelsey M. Kennedy, Lixin Chin, Andrea Curatolo, Alan Tien, Bruce Latham, Christobel M. Saunders, and David D. Sampson  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 7, pp. 2113-2124 (2014)

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We present optical coherence micro-elastography, an improved form of compression optical coherence elastography. We demonstrate the capacity of this technique to produce en face images, closely corresponding with histology, that reveal micro-scale mechanical contrast in human breast and lymph node tissues. We use phase-sensitive, three-dimensional optical coherence tomography (OCT) to probe the nanometer-to-micrometer-scale axial displacements in tissues induced by compressive loading. Optical coherence micro-elastography incorporates common-path interferometry, weighted averaging of the complex OCT signal and weighted least-squares regression. Using three-dimensional phase unwrapping, we have increased the maximum detectable strain eleven-fold over no unwrapping and the minimum detectable strain is 2.6 με. We demonstrate the potential of mechanical over optical contrast for visualizing micro-scale tissue structures in human breast cancer pathology and lymph node morphology.

© 2014 Optical Society of America

OCIS Codes
(110.1650) Imaging systems : Coherence imaging
(110.4500) Imaging systems : Optical coherence tomography
(100.5088) Image processing : Phase unwrapping

ToC Category:
Optical Coherence Tomography

Original Manuscript: April 9, 2014
Revised Manuscript: May 28, 2014
Manuscript Accepted: May 30, 2014
Published: June 9, 2014

Brendan F. Kennedy, Robert A. McLaughlin, Kelsey M. Kennedy, Lixin Chin, Andrea Curatolo, Alan Tien, Bruce Latham, Christobel M. Saunders, and David D. Sampson, "Optical coherence micro-elastography: mechanical-contrast imaging of tissue microstructure," Biomed. Opt. Express 5, 2113-2124 (2014)

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