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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 5, Iss. 8 — Aug. 1, 2014
  • pp: 2580–2590

Optimized approaches for optical sectioning and resolution enhancement in 2D structured illumination microscopy

Kevin O’Holleran and Michael Shaw  »View Author Affiliations

Biomedical Optics Express, Vol. 5, Issue 8, pp. 2580-2590 (2014)

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The use of structured illumination in fluorescence microscopy allows the suppression of out of focus light and an increase in effective spatial resolution. In this paper we consider different approaches for reconstructing 2D structured illumination images in order to combine these two attributes, to allow fast, optically sectioned, superresolution imaging. We present a linear reconstruction method that maximizes the axial frequency extent of the combined 2D structured illumination passband along with an empirically optimized approximation to this scheme. These reconstruction methods are compared to other schemes using structured illumination images of fluorescent samples. For sinusoidal excitation at half the incoherent cutoff frequency we find that removing information in the zero order passband except for a small region close to the excitation frequency, where it replaces the complementary information from the displaced first order passband, enables optimal reconstruction of optically sectioned images with enhanced spatial resolution.

© 2014 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: February 26, 2014
Revised Manuscript: April 8, 2014
Manuscript Accepted: April 28, 2014
Published: July 10, 2014

Kevin O’Holleran and Michael Shaw, "Optimized approaches for optical sectioning and resolution enhancement in 2D structured illumination microscopy," Biomed. Opt. Express 5, 2580-2590 (2014)

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