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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 1 — Aug. 2, 2010
  • pp: 236–245

Practical implementation of log-scale active illumination microscopy

Kengyeh K. Chu, Daryl Lim, and Jerome Mertz  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 1, pp. 236-245 (2010)
http://dx.doi.org/10.1364/BOE.1.000236


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Abstract

Active illumination microscopy (AIM) is a method of redistributing dynamic range in a scanning microscope using real-time feedback to control illumination power on a sub-pixel time scale. We describe and demonstrate a fully integrated instrument that performs both feedback and image reconstruction. The image is reconstructed on a logarithmic scale to accommodate the dynamic range benefits of AIM in a single output channel. A theoretical and computational analysis of the influence of noise on active illumination feedback is presented, along with imaging examples illustrating the benefits of AIM. While AIM is applicable to any type of scanning microscope, we apply it here specifically to two-photon microscopy.

© 2010 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Microscopy

History
Original Manuscript: May 28, 2010
Revised Manuscript: July 7, 2010
Manuscript Accepted: July 7, 2010
Published: July 16, 2010

Citation
Kengyeh K. Chu, Daryl Lim, and Jerome Mertz, "Practical implementation of log-scale active illumination microscopy," Biomed. Opt. Express 1, 236-245 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-1-236


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References

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