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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 1 — Aug. 2, 2010
  • pp: 246–259

Quantitative imaging of ischemic stroke through thinned skull in mice with Multi Exposure Speckle Imaging

Ashwin B. Parthasarathy, S. M. Shams Kazmi, and Andrew K. Dunn  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 1, pp. 246-259 (2010)
http://dx.doi.org/10.1364/BOE.1.000246


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Abstract

Laser Speckle Contrast Imaging (LSCI) has become a widely used technique to image cerebral blood flow in vivo. However, the quantitative accuracy of blood flow changes measured through the thin skull has not been investigated thoroughly. We recently developed a new Multi Exposure Speckle Imaging (MESI) technique to image blood flow while accounting for the effect of scattering from static tissue elements. In this paper we present the first in vivo demonstration of the MESI technique. The MESI technique was used to image the blood flow changes in a mouse cortex following photothrombotic occlusion of the middle cerebral artery. The Multi Exposure Speckle Imaging technique was found to accurately estimate flow changes due to ischemia in mice brains in vivo. These estimates of these flow changes were found to be unaffected by scattering from thinned skull.

© 2010 Optical Society of America

OCIS Codes
(120.6150) Instrumentation, measurement, and metrology : Speckle imaging
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

ToC Category:
Neuroscience and Brain Imaging

History
Original Manuscript: April 26, 2010
Revised Manuscript: June 28, 2010
Manuscript Accepted: July 1, 2010
Published: July 16, 2010

Citation
Ashwin B. Parthasarathy, S. M. Shams Kazmi, and Andrew K. Dunn, "Quantitative imaging of ischemic stroke through thinned skull in mice with Multi Exposure Speckle Imaging," Biomed. Opt. Express 1, 246-259 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-1-246


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