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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 1 — Aug. 2, 2010
  • pp: 285–294

Wound healing monitoring using near infrared fluorescent fibrinogen

Chia-Pin Pan, Yihui Shi, Khalid Amin, Charles S. Greenberg, Zishan Haroon, and Gregory W. Faris  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 1, pp. 285-294 (2010)

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We demonstrate a method for imaging the wound healing process with near infrared fluorescent fibrinogen. Wound healing studies were performed on a rat punch biopsy model. Fibrinogen was conjugated with a near infrared fluorescent dye and injected into the tail vein. Fibrinogen is a useful protein for tracking wound healing because it is involved in fibrin clot formation and formation of new provisional matrix through transglutaminase’s crosslinking activity. Strong fluorescence specific to the wound was observed and persisted for several days, indicating that the fibrinogen is converted to crosslinked fibrin. Administration of contrast agent simultaneously with wound creation led to primary labeling of the fibrin clot, indicating that the wound was in its early phase of healing. Administration on the following day showed labeling on the wound periphery, indicating location of formation of a new provisional matrix. This method may prove to be useful as a diagnostic for basic studies of the wound healing process, in drug development, or in clinical assessment of chronic wounds.

© 2010 OSA

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Molecular Imaging and Probe Development

Original Manuscript: June 16, 2010
Revised Manuscript: July 19, 2010
Manuscript Accepted: July 19, 2010
Published: July 27, 2010

Virtual Issues
Bio-Optics in Clinical Application, Nanotechnology, and Drug Discovery (2010) Biomedical Optics Express

Chia-Pin Pan, Yihui Shi, Khalid Amin, Charles S. Greenberg, Zishan Haroon, and Gregory W. Faris, "Wound healing monitoring using near infrared fluorescent fibrinogen," Biomed. Opt. Express 1, 285-294 (2010)

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