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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 1 — Aug. 2, 2010
  • pp: 87–96

Correction for specimen movement and rotation errors for in-vivo Optical Projection Tomography

Udo Jochen Birk, Matthias Rieckher, Nikos Konstantinides, Alex Darrell, Ana Sarasa-Renedo, Heiko Meyer, Nektarios Tavernarakis, and Jorge Ripoll  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 1, pp. 87-96 (2010)
http://dx.doi.org/10.1364/BOE.1.000087


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Abstract

The application of optical projection tomography to in-vivo experiments is limited by specimen movement during the acquisition. We present a set of mathematical correction methods applied to the acquired data stacks to correct for movement in both directions of the image plane. These methods have been applied to correct experimental data taken from in-vivo optical projection tomography experiments in Caenorhabditis elegans. Successful reconstructions for both fluorescence and white light (absorption) measurements are shown. Since no difference between movement of the animal and movement of the rotation axis is made, this approach at the same time removes artifacts due to mechanical drifts and errors in the assumed center of rotation.

© 2010 OSA

OCIS Codes
(100.6950) Image processing : Tomographic image processing
(170.6960) Medical optics and biotechnology : Tomography
(110.3010) Imaging systems : Image reconstruction techniques

ToC Category:
Image Reconstruction and Inverse Problems

History
Original Manuscript: May 28, 2010
Revised Manuscript: June 27, 2010
Manuscript Accepted: June 30, 2010
Published: July 14, 2010

Virtual Issues
Bio-Optics in Clinical Application, Nanotechnology, and Drug Discovery (2010) Biomedical Optics Express

Citation
Udo Jochen Birk, Matthias Rieckher, Nikos Konstantinides, Alex Darrell, Ana Sarasa-Renedo, Heiko Meyer, Nektarios Tavernarakis, and Jorge Ripoll, "Correction for specimen movement and rotation errors for in-vivo Optical Projection Tomography," Biomed. Opt. Express 1, 87-96 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-1-87


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