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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 1 — Aug. 2, 2010
  • pp: 97–105

Non-invasive surface-stripping for epifluorescence small animal imaging

Sophie Piper, Peyman Bahmani, Jan Klohs, Riad Bourayou, Peter Brunecker, Jochen Müller, Denise Harhausen, Ute Lindauer, Ulrich Dirnagl, Jens Steinbrink, and Andreas Wunder  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 1, pp. 97-105 (2010)
http://dx.doi.org/10.1364/BOE.1.000097


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Abstract

Non-invasive near-infrared fluorescence (NIRF) imaging is a powerful tool to study pathophysiology in a wide variety of animal disease models including brain diseases. However, especially in NIRF imaging of the brain or other deeper laying target sites, background fluorescence emitted from the scalp or superficial blood vessels can impede the detection of fluorescence in deeper tissue. Here, we introduce an effective method to reduce the impact of fluorescence from superficial layers. The approach uses excitation light at two different wavelengths generating two images with different depth sensitivities followed by an adapted subtraction algorithm. This technique leads to significant enhancement of the contrast and the detectability of fluorochromes located in deep tissue layers in tissue simulating phantoms and murine models with stroke.

© 2010 OSA

ToC Category:
Small Animal Imaging and Veterinary Studies

History
Original Manuscript: June 1, 2010
Revised Manuscript: June 24, 2010
Manuscript Accepted: July 2, 2010
Published: July 14, 2010

Virtual Issues
Bio-Optics in Clinical Application, Nanotechnology, and Drug Discovery (2010) Biomedical Optics Express

Citation
Sophie Piper, Peyman Bahmani, Jan Klohs, Riad Bourayou, Peter Brunecker, Jochen Müller, Denise Harhausen, Ute Lindauer, Ulrich Dirnagl, Jens Steinbrink, and Andreas Wunder, "Non-invasive surface-stripping for epifluorescence small animal imaging," Biomed. Opt. Express 1, 97-105 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-1-97


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