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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 2 — Sep. 1, 2010
  • pp: 385–397

SPLASSH: Open source software for camera-based high-speed, multispectral in-vivo optical image acquisition

Ryan Sun, Matthew B. Bouchard, and Elizabeth M. C. Hillman  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 2, pp. 385-397 (2010)

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Camera-based in-vivo optical imaging can provide detailed images of living tissue that reveal structure, function, and disease. High-speed, high resolution imaging can reveal dynamic events such as changes in blood flow and responses to stimulation. Despite these benefits, commercially available scientific cameras rarely include software that is suitable for in-vivo imaging applications, making this highly versatile form of optical imaging challenging and time-consuming to implement. To address this issue, we have developed a novel, open-source software package to control high-speed, multispectral optical imaging systems. The software integrates a number of modular functions through a custom graphical user interface (GUI) and provides extensive control over a wide range of inexpensive IEEE 1394 Firewire cameras. Multispectral illumination can be incorporated through the use of off-the-shelf light emitting diodes which the software synchronizes to image acquisition via a programmed microcontroller, allowing arbitrary high-speed illumination sequences. The complete software suite is available for free download. Here we describe the software’s framework and provide details to guide users with development of this and similar software.

© 2010 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Clinical Instrumentation

Original Manuscript: June 1, 2010
Revised Manuscript: July 28, 2010
Manuscript Accepted: July 28, 2010
Published: August 2, 2010

Virtual Issues
Optical Imaging and Spectroscopy (2010) Biomedical Optics Express

Ryan Sun, Matthew B. Bouchard, and Elizabeth M. C. Hillman, "SPLASSH: Open source software for camera-based high-speed, multispectral in-vivo optical image acquisition," Biomed. Opt. Express 1, 385-397 (2010)

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