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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 2 — Sep. 1, 2010
  • pp: 453–462

Folate receptor targeting silica nanoparticle probe for two-photon fluorescence bioimaging

Xuhua Wang, Sheng Yao, Hyo-Yang Ahn, Yuanwei Zhang, Mykhailo V. Bondar, Joseph A. Torres, and Kevin D. Belfield  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 2, pp. 453-462 (2010)

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Narrow dispersity organically modified silica nanoparticles (SiNPs), diameter ~30 nm, entrapping a hydrophobic two-photon absorbing fluorenyl dye, were synthesized by hydrolysis of triethoxyvinylsilane and (3-aminopropyl)triethoxysilane in the nonpolar core of Aerosol-OT micelles. The surface of the SiNPs were functionalized with folic acid, to specifically deliver the probe to folate receptor (FR) over-expressing Hela cells, making these folate two-photon dye-doped SiNPs potential candidates as probes for two-photon fluorescence microscopy (2PFM) bioimaging. In vitro studies using FR over-expressing Hela cells and low FR expressing MG63 cells demonstrated specific cellular uptake of the functionalized nanoparticles. One-photon fluorescence microscopy (1PFM) imaging, 2PFM imaging, and two-photon fluorescence lifetime microscopy (2P-FLIM) imaging of Hela cells incubated with folate-modified two-photon dye-doped SiNPs were demonstrated.

© 2010 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Nanotechnology and Plasmonics

Original Manuscript: June 15, 2010
Revised Manuscript: July 24, 2010
Manuscript Accepted: July 26, 2010
Published: August 2, 2010

Virtual Issues
Bio-Optics in Clinical Application, Nanotechnology, and Drug Discovery (2010) Biomedical Optics Express
September 10, 2010 Spotlight on Optics

Xuhua Wang, Sheng Yao, Hyo-Yang Ahn, Yuanwei Zhang, Mykhailo V. Bondar, Joseph A. Torres, and Kevin D. Belfield, "Folate receptor targeting silica nanoparticle probe for two-photon fluorescence bioimaging," Biomed. Opt. Express 1, 453-462 (2010)

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