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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 2 — Sep. 1, 2010
  • pp: 482–488

Linear diode laser bar optical stretchers for cell deformation

Ihab Sraj, David W.M. Marr, and Charles D. Eggleton  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 2, pp. 482-488 (2010)

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To investigate the use of linear diode laser bars to optically stretch cells and measure their mechanical properties, we present numerical simulations using the immersed boundary method (IBM) coupled with classic ray optics. Cells are considered as three-dimensional (3D) spherical elastic capsules immersed in a fluid subjected to both optical and hydrodynamic forces in a periodic domain. We simulate cell deformation induced by both single and dual diode laser bar configurations and show that a single diode laser bar induces significant stretching but also induces cell translation of speed < 10 µm/sec for applied 6.6 mW/µm power in unconfined systems. The dual diode laser bar configuration, however, can be used to both stretch and optically trap cells at a fixed position. The net cell deformation was found to be a function of the total laser power and not the power distribution between single or dual diode laser bar configurations.

© 2010 OSA

OCIS Codes
(000.4430) General : Numerical approximation and analysis
(140.2020) Lasers and laser optics : Diode lasers
(140.7010) Lasers and laser optics : Laser trapping
(170.1530) Medical optics and biotechnology : Cell analysis

ToC Category:
Optical Traps, Manipulation, and Tracking

Original Manuscript: June 1, 2010
Revised Manuscript: July 31, 2010
Manuscript Accepted: August 2, 2010
Published: August 5, 2010

Virtual Issues
Bio-Optics in Clinical Application, Nanotechnology, and Drug Discovery (2010) Biomedical Optics Express

Ihab Sraj, David W. M. Marr, and Charles D. Eggleton, "Linear diode laser bar optical stretchers for cell deformation," Biomed. Opt. Express 1, 482-488 (2010)

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