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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 2 — Sep. 1, 2010
  • pp: 574–586

Instrumentation to rapidly acquire fluorescence wavelength-time matrices of biological tissues

William R. Lloyd, Robert H. Wilson, Ching-Wei Chang, Gregory D. Gillispie, and Mary-Ann Mycek  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 2, pp. 574-586 (2010)

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A fiber-optic system was developed to rapidly acquire tissue fluorescence wavelength-time matrices (WTMs) with high signal-to-noise ratio (SNR). The essential system components (473 nm microchip laser operating at 3 kHz repetition frequency, fiber-probe assemblies, emission monochromator, photomultiplier tube, and digitizer) were assembled into a compact and clinically-compatible unit. Data were acquired from fluorescence standards and tissue-simulating phantoms to test system performance. Fluorescence decay waveforms with SNR > 100 at the decay curve peak were obtained in less than 30 ms. With optimized data transfer and monochromator stepping functions, it should be feasible to acquire a full WTM at 5 nm emission wavelength intervals over a 200 nm range in under 2 seconds.

© 2010 OSA

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Spectroscopic Diagnostics

Original Manuscript: June 16, 2010
Revised Manuscript: August 6, 2010
Manuscript Accepted: August 7, 2010
Published: August 10, 2010

Virtual Issues
Optical Imaging and Spectroscopy (2010) Biomedical Optics Express

William R. Lloyd, Robert H. Wilson, Ching-Wei Chang, Gregory D. Gillispie, and Mary-Ann Mycek, "Instrumentation to rapidly acquire fluorescence wavelength-time matrices of biological tissues," Biomed. Opt. Express 1, 574-586 (2010)

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