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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 2 — Sep. 1, 2010
  • pp: 598–609

Implementation of a continuous scanning procedure and a line scan camera for thin-sheet laser imaging microscopy

Peter Schacht, Shane B. Johnson, and Peter A. Santi  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 2, pp. 598-609 (2010)

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We report development of a continuous scanning procedure and the use of a time delay integration (TDI) line scan camera for a light-sheet based microscope called a thin-sheet laser imaging microscope (TSLIM). TSLIM is an optimized version of a light-sheet fluorescent microscope that previously used a start/stop scanning procedure to move the specimen through the thinnest portion of a light-sheet and stitched the image columns together to produce a well-focused composite image. In this paper, hardware and software enhancements to TSLIM are described that allow for dual sided, dual illumination lasers, and continuous scanning of the specimen using either a full-frame CCD camera and a TDI line scan camera. These enhancements provided a ~70% reduction in the time required for composite image generation and a ~63% reduction in photobleaching of the specimen compared to the start/stop procedure.

© 2010 OSA

OCIS Codes
(040.1490) Detectors : Cameras
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(220.0220) Optical design and fabrication : Optical design and fabrication

ToC Category:

Original Manuscript: July 15, 2010
Revised Manuscript: August 6, 2010
Manuscript Accepted: August 11, 2010
Published: August 13, 2010

Peter Schacht, Shane B. Johnson, and Peter A. Santi, "Implementation of a continuous scanning procedure and a line scan camera for thin-sheet laser imaging microscopy," Biomed. Opt. Express 1, 598-609 (2010)

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  1. P. A. Santi, S. B. Johnson, M. Hillenbrand, P. Z. GrandPre, T. J. Glass, and J. R. Leger, “Thin-sheet laser imaging microscopy for optical sectioning of thick tissues,” Biotechniques 46(4), 287–294 (2009). [PubMed]
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