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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 2 — Sep. 1, 2010
  • pp: 720–728

Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters

Robert M. Pasternack, Bryan Rabin, Jing-Yi Zheng, and Nada N. Boustany  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 2, pp. 720-728 (2010)
http://dx.doi.org/10.1364/BOE.1.000720


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Abstract

We demonstrate an optical Fourier filtering method which can be used to characterize subcellular morphology during dynamic cellular function. In this paper, our Fourier filters were based on two-dimensional Gabor elementary functions, which can be tuned to sense directly object size and orientation. We utilize this method to quantify changes in mitochondrial and nuclear structure during the first three hours of apoptosis. We find that the technique is sensitive to a decrease in particle orientation consistent with apoptosis-induced mitochondrial fragmentation. The scattering signal changes were less pronounced in the nucleus and the remainder of the cytoplasm. Particles in these regions were less oriented than mitochondria and did not change orientation significantly.

© 2010 OSA

OCIS Codes
(070.1170) Fourier optics and signal processing : Analog optical signal processing
(170.1530) Medical optics and biotechnology : Cell analysis
(290.5820) Scattering : Scattering measurements

ToC Category:
Cell Studies

History
Original Manuscript: July 6, 2010
Revised Manuscript: August 21, 2010
Manuscript Accepted: August 21, 2010
Published: August 25, 2010

Virtual Issues
Optical Imaging and Spectroscopy (2010) Biomedical Optics Express

Citation
Robert M. Pasternack, Bryan Rabin, Jing-Yi Zheng, and Nada N. Boustany, "Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters [Invited]," Biomed. Opt. Express 1, 720-728 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-2-720


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