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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 3 — Oct. 1, 2010
  • pp: 1014–1025

Detection of colonic inflammation with Fourier transform infrared spectroscopy using a flexible silver halide fiber

Vinay K. Katukuri, John Hargrove, Sharon J. Miller, Kinan Rahal, John Y. Kao, Rolf Wolters, Ellen M. Zimmermann, and Thomas D. Wang  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 3, pp. 1014-1025 (2010)

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Persistent colonic inflammation increases risk for cancer, but mucosal appearance on conventional endoscopy correlates poorly with histology. Here we demonstrate the use of a flexible silver halide fiber to collect mid-infrared absorption spectra and an interval model to distinguish colitis from normal mucosa in dextran sulfate sodium treated mice. The spectral regime between 950 and 1800 cm−1 was collected from excised colonic specimens and compared with histology. Our model identified 3 sub-ranges that optimize the classification results, and the performance for detecting inflammation resulted in a sensitivity, specificity, accuracy, and positive predictive value of 92%, 88%, 90%, and 88%, respectively.

© 2010 OSA

OCIS Codes
(070.4790) Fourier optics and signal processing : Spectrum analysis
(070.5010) Fourier optics and signal processing : Pattern recognition
(110.2350) Imaging systems : Fiber optics imaging
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(300.6300) Spectroscopy : Spectroscopy, Fourier transforms

ToC Category:
Optics in Cancer Research

Original Manuscript: July 30, 2010
Revised Manuscript: September 16, 2010
Manuscript Accepted: September 19, 2010
Published: September 21, 2010

Vinay K. Katukuri, John Hargrove, Sharon J. Miller, Kinan Rahal, John Y. Kao, Rolf Wolters, Ellen M. Zimmermann, and Thomas D. Wang, "Detection of colonic inflammation with Fourier transform infrared spectroscopy using a flexible silver halide fiber," Biomed. Opt. Express 1, 1014-1025 (2010)

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  1. J. Xie and S. H. Itzkowitz, “Cancer in inflammatory bowel disease,” World J. Gastroenterol. 14(3), 378–389 (2008). [CrossRef] [PubMed]
  2. T. Ullman, V. Croog, N. Harpaz, D. Sachar, and S. Itzkowitz, “Progression of flat low-grade dysplasia to advanced neoplasia in patients with ulcerative colitis,” Gastroenterology 125(5), 1311–1319 (2003). [CrossRef] [PubMed]
  3. R. B. Gupta, N. Harpaz, S. Itzkowitz, S. Hossain, S. Matula, A. Kornbluth, C. Bodian, and T. Ullman, “Histologic inflammation is a risk factor for progression to colorectal neoplasia in ulcerative colitis: a cohort study,” Gastroenterology 133(4), 1099–1105, quiz 1340–1341 (2007). [CrossRef] [PubMed]
  4. M. Rutter, B. Saunders, K. Wilkinson, S. Rumbles, G. Schofield, M. Kamm, C. Williams, A. Price, I. Talbot, and A. Forbes, “Severity of inflammation is a risk factor for colorectal neoplasia in ulcerative colitis,” Gastroenterology 126(2), 451–459 (2004). [CrossRef] [PubMed]
  5. P. Gomes, C. du Boulay, C. L. Smith, and G. Holdstock, “Relationship between disease activity indices and colonoscopic findings in patients with colonic inflammatory bowel disease,” Gut 27(1), 92–95 (1986). [CrossRef] [PubMed]
  6. T. D. Wang, G. Triadafilopoulos, J. M. Crawford, L. R. Dixon, T. Bhandari, P. Sahbaie, S. Friedland, R. Soetikno, and C. H. Contag, “Detection of endogenous biomolecules in Barrett’s esophagus by Fourier transform infrared spectroscopy,” Proc. Natl. Acad. Sci. U.S.A. 104(40), 15864–15869 (2007). [CrossRef] [PubMed]
  7. N. Fujioka, Y. Morimoto, T. Arai, and M. Kikuchi, “Discrimination between normal and malignant human gastric tissues by Fourier transform infrared spectroscopy,” Cancer Detect. Prev. 28(1), 32–36 (2004). [CrossRef] [PubMed]
  8. S. G. El-Tawil, R. Adnan, Z. N. Muhamed, and N. H. Othman, “Comparative study between Pap smear cytology and FTIR spectroscopy: a new tool for screening for cervical cancer,” Pathology 40(6), 600–603 (2008). [CrossRef] [PubMed]
  9. Q. B. Li, Z. Xu, N. W. Zhang, L. Zhang, F. Wang, L. M. Yang, J. S. Wang, S. Zhou, Y. F. Zhang, X. S. Zhou, J. S. Shi, and J. G. Wu, “In vivo and in situ detection of colorectal cancer using Fourier transform infrared spectroscopy,” World J. Gastroenterol. 11(3), 327–330 (2005). [PubMed]
  10. B. Rigas, S. Morgello, I. S. Goldman, and P. T. Wong, “Human colorectal cancers display abnormal Fourier-transform infrared spectra,” Proc. Natl. Acad. Sci. U.S.A. 87(20), 8140–8144 (1990). [CrossRef] [PubMed]
  11. D. C. Fernandez, R. Bhargava, S. M. Hewitt, and I. W. Levin, “Infrared spectroscopic imaging for histopathologic recognition,” Nat. Biotechnol. 23(4), 469–474 (2005). [CrossRef] [PubMed]
  12. C. Charlton, A. Katzir, and B. Mizaikoff, “Infrared evanescent field sensing with quantum cascade lasers and planar silver halide waveguides,” Anal. Chem. 77(14), 4398–4403 (2005). [CrossRef] [PubMed]
  13. U. Bindig, M. Meinke, I. Gersonde, O. Spector, I. Vasserman, A. Katzir, and G. Müller, “IR-biosensor: flat silver halide fiber for bio-medical sensing?” Sens. Actuators B Chem. 74(1-3), 37–46 (2001). [CrossRef]
  14. M. A. Mackanos, J. Hargrove, R. Wolters, C. B. Du, S. Friedland, R. M. Soetikno, C. H. Contag, M. R. Arroyo, J. M. Crawford, and T. D. Wang, “Use of an endoscope-compatible probe to detect colonic dysplasia with Fourier transform infrared spectroscopy,” J. Biomed. Opt. 14(4), 044006 (2009). [CrossRef] [PubMed]
  15. S. Wirtz, C. Neufert, B. Weigmann, and M. F. Neurath, “Chemically induced mouse models of intestinal inflammation,” Nat. Protoc. 2(3), 541–546 (2007). [CrossRef] [PubMed]
  16. S. Fujii, T. Fujimori, H. Kawamata, J. Takeda, K. Kitajima, F. Omotehara, T. Kaihara, T. Kusaka, K. Ichikawa, Y. Ohkura, Y. Ono, J. Imura, S. Yamaoka, C. Sakamoto, Y. Ueda, and T. Chiba, “Development of colonic neoplasia in p53 deficient mice with experimental colitis induced by dextran sulphate sodium,” Gut 53(5), 710–716 (2004). [CrossRef] [PubMed]
  17. H. S. Cooper, L. Everley, W. C. Chang, G. Pfeiffer, B. Lee, S. Murthy, and M. L. Clapper, “The role of mutant Apc in the development of dysplasia and cancer in the mouse model of dextran sulfate sodium-induced colitis,” Gastroenterology 121(6), 1407–1416 (2001). [CrossRef] [PubMed]
  18. S. Wold, A. Ruhe, H. Wold, and W. Dunn, “The Collinearity Problem in Linear-Regression - the Partial Least-Squares (PLS) Approach to Generalized Inverses,” SIAM J. Sci. Stat. Comput. 5(3), 735–743 (1984). [CrossRef]
  19. P. Geladi and B. Kowalski, “Partial Least-Squares Regression - a Tutorial,” Anal. Chim. Acta 185(1), 1–17 (1986). [CrossRef]
  20. S. de Jong, “SIMPLS - an Alternative Approach to Partial Least-Squares Regression,” Chemom. Intell. Lab. Syst. 18(3), 251–263 (1993). [CrossRef]
  21. B. Wise, N. Gallagher, R. Bro, J. Shaver, W. Windig, and R. Koch, PLS_Toolbox Version 4.0 Manual (2006).
  22. A. Savitzky and M. J. E. Golay, “Smoothing and Differentiation of Data by Simplified Least Squares Procedures,” Anal. Chem. 36(8), 1627–1639 (1964). [CrossRef]
  23. I. Chong and C. Jun, “Performance of some variable selection methods when multicollinearity is present,” Chemom. Intell. Lab. Syst. 78(1-2), 103–112 (2005). [CrossRef]
  24. D. J. Law, E. M. Labut, R. D. Adams, and J. L. Merchant, “An isoform of ZBP-89 predisposes the colon to colitis,” Nucleic Acids Res. 34(5), 1342–1350 (2006). [CrossRef] [PubMed]
  25. M. L. Mutinga, R. D. Odze, H. H. Wang, J. L. Hornick, and F. A. Farraye, “The clinical significance of right-sided colonic inflammation in patients with left-sided chronic ulcerative colitis,” Inflamm. Bowel Dis. 10(3), 215–219 (2004). [CrossRef] [PubMed]
  26. S. Argov, R. K. Sahu, E. Bernshtain, A. Salman, G. Shohat, U. Zelig, and S. Mordechai, “Inflammatory bowel diseases as an intermediate stage between normal and cancer: a FTIR-microspectroscopy approach,” Biopolymers 75(5), 384–392 (2004). [CrossRef] [PubMed]
  27. M. Seo, M. Okada, T. Yao, M. Ueki, S. Arima, and M. Okumura, “An index of disease activity in patients with ulcerative colitis,” Am. J. Gastroenterol. 87(8), 971–976 (1992). [PubMed]
  28. R. Bhargava, D. C. Fernandez, S. M. Hewitt, and I. W. Levin, “High throughput assessment of cells and tissues: Bayesian classification of spectral metrics from infrared vibrational spectroscopic imaging data,” Biochim. Biophys. Acta 1758(7), 830–845 (2006). [CrossRef] [PubMed]
  29. R. Bhargava, “Towards a practical Fourier transform infrared chemical imaging protocol for cancer histopathology,” Anal. Bioanal. Chem. 389(4), 1155–1169 (2007). [CrossRef] [PubMed]
  30. M. J. German, A. Hammiche, N. Ragavan, M. J. Tobin, L. J. Cooper, S. S. Matanhelia, A. C. Hindley, C. M. Nicholson, N. J. Fullwood, H. M. Pollock, and F. L. Martin, “Infrared spectroscopy with multivariate analysis potentially facilitates the segregation of different types of prostate cell,” Biophys. J. 90(10), 3783–3795 (2006). [CrossRef] [PubMed]
  31. M. M. Patel, J. D. Smart, T. G. Nevell, R. J. Ewen, P. J. Eaton, and J. Tsibouklis, “Mucin/poly(acrylic acid) interactions: a spectroscopic investigation of mucoadhesion,” Biomacromolecules 4(5), 1184–1190 (2003). [CrossRef] [PubMed]
  32. E. Bogomolny, M. Huleihel, Y. Suproun, R. K. Sahu, and S. Mordechai, “Early spectral changes of cellular malignant transformation using Fourier transform infrared microspectroscopy,” J. Biomed. Opt. 12(2), 024003 (2007). [CrossRef] [PubMed]

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