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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 3 — Oct. 1, 2010
  • pp: 1026–1037

High-speed focal modulation microscopy using acousto-optical modulators

Shau Poh Chong, Chee Howe Wong, Kit Fei Wong, Colin J.R. Sheppard, and Nanguang Chen  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 3, pp. 1026-1037 (2010)
http://dx.doi.org/10.1364/BOE.1.001026


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Abstract

Focal Modulation Microscopy (FMM) is a single-photon excitation fluorescence microscopy technique which effectively rejects the out-of-focus fluorescence background that arises when imaging deep inside biological tissues. Here, we report on the implementation of FMM in which laser intensity modulation at the focal plane is achieved using acousto-optic modulators (AOM). The modulation speed is greatly enhanced to the MHz range and thus enables real-time image acquisition. The capability of FMM is demonstrated by imaging fluorescence labeled vasculatures in mouse brain as well as self-made tissue phantom.

© 2010 OSA

OCIS Codes
(120.4570) Instrumentation, measurement, and metrology : Optical design of instruments
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.2520) Microscopy : Fluorescence microscopy
(110.0113) Imaging systems : Imaging through turbid media

ToC Category:
Microscopy

History
Original Manuscript: May 25, 2010
Revised Manuscript: July 19, 2010
Manuscript Accepted: September 23, 2010
Published: September 30, 2010

Virtual Issues
Advances in Optical Coherence Tomography, Photoacoustic Imaging, and Microscopy (2010) Biomedical Optics Express

Citation
Shau Poh Chong, Chee Howe Wong, Kit Fei Wong, Colin J.R. Sheppard, and Nanguang Chen, "High-speed focal modulation microscopy using acousto-optical modulators," Biomed. Opt. Express 1, 1026-1037 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-3-1026


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