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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 3 — Oct. 1, 2010
  • pp: 762–770

Bacteria detection with thin wetting film lensless imaging

C. P. Allier, G. Hiernard, V Poher, and J. M. Dinten  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 3, pp. 762-770 (2010)

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Lensless on-chip imaging is a promising technique to count and monitor cells and micro-objects in liquid sample. In this paper we apply this technique to the observation of µL sample containing bacteria evaporated onto a microscope slide. Compared with previously reported techniques, a large improvement in signal to noise ratio is obtained due to the presence of a few μm thick wetting film creating a micro-lens on top of each bacteria. In these conditions, standard CMOS sensor are able to detect micro-objects as small as few μm, e.g. E.coli and Bacillus subtilis bacteria and 1 μm polymer beads with a large signal to noise ratio of 45 ± 10. An overall detection efficiency of 85 ± 7% and a co-localization error of σ1D = 1.1μm compared with reference fluorescence microscopy images are achieved. This novel technique will be used as a pre-positioning tool prior to other optical identification methods, e.g. Raman spectroscopy.

© 2010 OSA

OCIS Codes
(110.2970) Imaging systems : Image detection systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:

Original Manuscript: July 20, 2010
Revised Manuscript: August 10, 2010
Manuscript Accepted: August 10, 2010
Published: August 31, 2010

C. P. Allier, G. Hiernard, V. Poher, and J. M. Dinten, "Bacteria detection with thin wetting film lensless imaging," Biomed. Opt. Express 1, 762-770 (2010)

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  1. C. Kirschner, K. Maquelin, P. Pina, N. A. Ngo Thi, L. P. Choo-Smith, G. D. Sockalingum, C. Sandt, D. Ami, F. Orsini, S. M. Doglia, P. Allouch, M. Mainfait, G. J. Puppels, and D. Naumann, “Classification and identification of enterococci: a comparative phenotypic, genotypic, and vibrational spectroscopic study,” J. Clin. Microbiol. 39(5), 1763–1770 (2001). [CrossRef] [PubMed]
  2. R. Goodacre, E. M. Timmins, R. Burton, N. Kaderbhai, A. M. Woodward, D. B. Kell, and P. J. Rooney, “Rapid identification of urinary tract infection bacteria using hyperspectral whole-organism fingerprinting and artificial neural networks,” Microbiology 144(5), 1157–1170 (1998). [CrossRef] [PubMed]
  3. M. Harz, M. Kiehntopf, S. Stöckel, P. Rösch, E. Straube, T. Deufel, and J. Popp, “Direct analysis of clinical relevant single bacterial cells from cerebrospinal fluid during bacterial meningitis by means of micro-Raman spectroscopy,” J Biophotonics 2(1-2), 70–80 (2009). [CrossRef] [PubMed]
  4. J. Guicheteau, S. Christesen, D. Emge, and A. Tripathi, “Bacterial mixture identification using Raman and surface-enhanced Raman chemical imaging,” J. Raman Spectrosc. (2010), to be published. [CrossRef]
  5. R. Mariella., “Sample preparation: the weak link in microfluidics-based biodetection,” Biomed. Microdevices 10(6), 777–784 (2008). [CrossRef] [PubMed]
  6. T. W. Su, S. Seo, A. Erlinger, and A. Ozcan, “High-throughput lensfree imaging and characterization of a heterogeneous cell solution on a chip,” Biotechnol. Bioeng. 102(3), 856–868 (2009). [CrossRef] [PubMed]
  7. S. Seo, T. W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, “Lensfree holographic imaging for on-chip cytometry and diagnostics,” Lab Chip 9(6), 777–787 (2009). [CrossRef] [PubMed]
  8. O. Mudanyali, A. Erlinger, S. Seo, T.-W. Su, D. Tseng, and A. Ozcan, “Lensless on-chip imaging of cells provides a new tool for high-throughput cell-biology and medical diagnostics,” J. Vis. Exp. 34, (2009). [PubMed]
  9. M.-S. Kim, T. Scharf, and H. P. Herzig, “Small-size microlens characterization by multiwavelength high-resolution interference microscopy,” Opt. Express 18(14), 14319–14329 (2010). [CrossRef] [PubMed]
  10. C. Oh, S. O. Isikman, B. Khademhosseinieh, and A. Ozcan, “On-chip differential interference contrast microscopy using lensless digital holography,” Opt. Express 18(5), 4717–4726 (2010). [CrossRef] [PubMed]

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