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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 3 — Oct. 1, 2010
  • pp: 762–770

Bacteria detection with thin wetting film lensless imaging

C. P. Allier, G. Hiernard, V Poher, and J. M. Dinten  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 3, pp. 762-770 (2010)
http://dx.doi.org/10.1364/BOE.1.000762


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Abstract

Lensless on-chip imaging is a promising technique to count and monitor cells and micro-objects in liquid sample. In this paper we apply this technique to the observation of µL sample containing bacteria evaporated onto a microscope slide. Compared with previously reported techniques, a large improvement in signal to noise ratio is obtained due to the presence of a few μm thick wetting film creating a micro-lens on top of each bacteria. In these conditions, standard CMOS sensor are able to detect micro-objects as small as few μm, e.g. E.coli and Bacillus subtilis bacteria and 1 μm polymer beads with a large signal to noise ratio of 45 ± 10. An overall detection efficiency of 85 ± 7% and a co-localization error of σ1D = 1.1μm compared with reference fluorescence microscopy images are achieved. This novel technique will be used as a pre-positioning tool prior to other optical identification methods, e.g. Raman spectroscopy.

© 2010 OSA

OCIS Codes
(110.2970) Imaging systems : Image detection systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Microscopy

History
Original Manuscript: July 20, 2010
Revised Manuscript: August 10, 2010
Manuscript Accepted: August 10, 2010
Published: August 31, 2010

Citation
C. P. Allier, G. Hiernard, V. Poher, and J. M. Dinten, "Bacteria detection with thin wetting film lensless imaging," Biomed. Opt. Express 1, 762-770 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-3-762


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References

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