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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 3 — Oct. 1, 2010
  • pp: 791–797

High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process

Keisuke Isobe, Akira Suda, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 3, pp. 791-797 (2010)

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We demonstrate high-resolution fluorescence microscopy based on a cyclic sequential multiphoton (CSM) process, which gives rise to fluorescence emission following a sequence of cyclic transitions between the bright and dark states of a fluorophore induced by pump and reverse light. By temporally modulating the reverse intensity, we can extract the fluorescence signal generated through the CSM process. We show that the demodulated fluorescence signal is nonlinearly proportional to the excitation intensities and it gives a higher spatial resolution than that of a confocal microscope.

© 2010 OSA

OCIS Codes
(120.1880) Instrumentation, measurement, and metrology : Detection
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: August 4, 2010
Revised Manuscript: August 19, 2010
Manuscript Accepted: September 2, 2010
Published: September 7, 2010

Keisuke Isobe, Akira Suda, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa, "High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process," Biomed. Opt. Express 1, 791-797 (2010)

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