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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 3 — Oct. 1, 2010
  • pp: 798–811

Measurement of absolute blood flow velocity in outflow tract of HH18 chicken embryo based on 4D reconstruction using spectral domain optical coherence tomography

Zhenhe Ma, Aiping Liu, Xin Yin, Aaron Troyer, Kent Thornburg, Ruikang K. Wang, and Sandra Rugonyi  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 3, pp. 798-811 (2010)

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The measurement of blood-plasma absolute velocity distributions with high spatial and temporal resolution in vivo is important for the investigation of embryonic heart at its early stage of development. We introduce a novel method to measure absolute blood flow velocity based on high speed spectral domain optical coherence tomography (OCT) and apply it to measure velocities across the heart outflow tract (OFT) of a chicken embryo (stage HH18). First, we use the OCT system to acquire 4D [(x,y,z) + t] images of the OFT in vivo. Second, we reconstruct the 4D microstructural images and obtain the orientation of the OFT at its maximum expansion, from which the centerline of the OFT is calculated based on the OFT boundary segmentation. Assuming flow is parallel to the vessel orientation, the obtained centerline indicates the flow direction. Finally, the absolute flow velocity is evaluated based on the direction given by the centerline and the axial velocity obtained from Doppler OCT. Using this method, we compare flow velocity profiles at various positions along the chicken embryo OFT.

© 2010 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(110.4155) Imaging systems : Multiframe image processing

ToC Category:
Optical Coherence Tomography

Original Manuscript: July 12, 2010
Revised Manuscript: August 16, 2010
Manuscript Accepted: September 7, 2010
Published: September 8, 2010

Zhenhe Ma, Aiping Liu, Xin Yin, Aaron Troyer, Kent Thornburg, Ruikang K. Wang, and Sandra Rugonyi, "Measurement of absolute blood flow velocity in outflow tract of HH18 chicken embryo based on 4D reconstruction using spectral domain optical coherence tomography," Biomed. Opt. Express 1, 798-811 (2010)

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  1. J. R. Hove, R. W. Köster, A. S. Forouhar, G. Acevedo-Bolton, S. E. Fraser, and M. Gharib, “Intracardiac fluid forces are an essential epigenetic factor for embryonic cardiogenesis,” Nature 421(6919), 172–177 (2003). [CrossRef] [PubMed]
  2. N. T. Ursem, S. Stekelenburg-de Vos, J. W. Wladimiroff, R. E. Poelmann, A. C. Gittenberger-de Groot, N. Hu, and E. B. Clark, “Ventricular diastolic filling characteristics in stage-24 chick embryos after extra-embryonic venous obstruction,” J. Exp. Biol. 207(9), 1487–1490 (2004). [CrossRef] [PubMed]
  3. A. F. M. Moorman and V. M. Christoffels, “Cardiac chamber formation: development, genes, and evolution,” Physiol. Rev. 83(4), 1223–1267 (2003). [PubMed]
  4. K. Ruijtenbeek, J. G. R. De Mey, C. E. Blanco, and H. Ehmke, “The chicken embryo in developmental physiology of the cardiovascular system: a traditional model with new possibilities,” Am. J. Physiol. Regul. Integr. Comp. Physiol. 283(2), R549–R550, author reply R550–R551 (2002). [PubMed]
  5. V. Hamburger and H. L. Hamilton, “A series of normal stages in the development of the chick embryo,” J. Morphol. 88(1), 49–92 (1951). [CrossRef]
  6. A. C. Gittenberger-de Groot, M. M. Bartelings, M. C. Deruiter, and R. E. Poelmann, “Basics of cardiac development for the understanding of congenital heart malformations,” Pediatr. Res. 57(2), 169–176 (2005). [CrossRef] [PubMed]
  7. C. P. Wang, “Laser Doppler velocimetry,” J. Quant. Spectrosc. Radiat. Transf. 40(3), 309–319 (1988). [CrossRef]
  8. M. W. Jenkins, O. Q. Chughtai, A. N. Basavanhally, M. Watanabe, and A. M. Rollins, “In vivo gated 4D imaging of the embryonic heart using optical coherence tomography,” J. Biomed. Opt. 12(3), 030505 (2007). [CrossRef] [PubMed]
  9. F. S. Foster, M. Y. Zhang, Y. Q. Zhou, G. Liu, J. Mehi, E. Cherin, K. A. Harasiewicz, B. G. Starkoski, L. Zan, D. A. Knapik, and S. L. Adamson, “A new ultrasound instrument for in vivo microimaging of mice,” Ultrasound Med. Biol. 28(9), 1165–1172 (2002). [CrossRef] [PubMed]
  10. T. C. McQuinn, M. Bratoeva, A. Dealmeida, M. Remond, R. P. Thompson, and D. Sedmera, “High-frequency ultrasonographic imaging of avian cardiovascular development,” Dev. Dyn. 236(12), 3503–3513 (2007). [CrossRef] [PubMed]
  11. C. K. Phoon, O. Aristizabal, and D. H. Turnbull, “40 MHz Doppler characterization of umbilical and dorsal aortic blood flow in the early mouse embryo,” Ultrasound Med. Biol. 26(8), 1275–1283 (2000). [CrossRef] [PubMed]
  12. C. K. Phoon, O. Aristizábal, and D. H. Turnbull, “Spatial velocity profile in mouse embryonic aorta and Doppler-derived volumetric flow: a preliminary model,” Am. J. Physiol. Heart Circ. Physiol. 283(3), H908–H916 (2002). [PubMed]
  13. P. Vennemann, K. T. Kiger, R. Lindken, B. C. Groenendijk, S. Stekelenburg-de Vos, T. L. ten Hagen, N. T. Ursem, R. E. Poelmann, J. Westerweel, and B. P. Hierck, “In vivo micro particle image velocimetry measurements of blood-plasma in the embryonic avian heart,” J. Biomech. 39(7), 1191–1200 (2006). [CrossRef] [PubMed]
  14. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991). [CrossRef] [PubMed]
  15. T. M. Yelbuz, M. A. Choma, L. Thrane, M. L. Kirby, and J. A. Izatt, “Optical coherence tomography: a new high-resolution imaging technology to study cardiac development in chick embryos,” Circulation 106(22), 2771–2774 (2002). [CrossRef] [PubMed]
  16. V. X. D. Yang, M. L. Gordon, E. Seng-Yue, S. Lo, B. Qi, J. Pekar, A. Mok, B. C. Wilson, and I. A. Vitkin, “High speed, wide velocity dynamic range Doppler optical coherence tomography (Part II): Imaging in vivo cardiac dynamics of Xenopus laevis,” Opt. Express 11(14), 1650–1658 (2003). [CrossRef] [PubMed]
  17. R. Leitgeb, C. K. Hitzenberger, and A. F. Fercher, “Performance of fourier domain vs. time domain optical coherence tomography,” Opt. Express 11(8), 889–894 (2003). [CrossRef] [PubMed]
  18. M. A. Choma, M. Sarunic, C. Yang, and J. Izatt, “Sensitivity advantage of swept source and Fourier domain optical coherence tomography,” Opt. Express 11(18), 2183–2189 (2003). [CrossRef] [PubMed]
  19. J. F. de Boer, B. Cense, B. H. Park, M. C. Pierce, G. J. Tearney, and B. E. Bouma, “Improved signal-to-noise ratio in spectral-domain compared with time-domain optical coherence tomography,” Opt. Lett. 28(21), 2067–2069 (2003). [CrossRef] [PubMed]
  20. R. Huber, D. C. Adler, and J. G. Fujimoto, “Buffered Fourier domain mode locking: Unidirectional swept laser sources for optical coherence tomography imaging at 370,000 lines/s,” Opt. Lett. 31(20), 2975–2977 (2006). [CrossRef] [PubMed]
  21. A. M. Davis, F. G. Rothenberg, N. Shepherd, and J. A. Izatt, “In vivo spectral domain optical coherence tomography volumetric imaging and spectral Doppler velocimetry of early stage embryonic chicken heart development,” J. Opt. Soc. Am. A 25(12), 3134–3143 (2008). [CrossRef] [PubMed]
  22. A. Mariampillai, B. A. Standish, N. R. Munce, C. Randall, G. Liu, J. Y. Jiang, A. E. Cable, I. A. Vitkin, and V. X. D. Yang, “Doppler optical cardiogram gated 2D color flow imaging at 1000 fps and 4D in vivo visualization of embryonic heart at 45 fps on a swept source OCT system,” Opt. Express 15(4), 1627–1638 (2007). [CrossRef] [PubMed]
  23. M. Liebling, A. S. Forouhar, M. Gharib, S. E. Fraser, and M. E. Dickinson, “Four-dimensional cardiac imaging in living embryos via postacquisition synchronization of nongated slice sequences,” J. Biomed. Opt. 10(5), 054001 (2005). [CrossRef] [PubMed]
  24. A. Liu, R. K. Wang, K. L. Thornburg, and S. Rugonyi, “Efficient postacquisition synchronization of 4-D nongated cardiac images obtained from optical coherence tomography: application to 4-D reconstruction of the chick embryonic heart,” J. Biomed. Opt. 14(4), 044020 (2009). [CrossRef] [PubMed]
  25. J. Zhang and Z. Chen, “In vivo blood flow imaging by a swept laser source based Fourier domain optical Doppler tomography,” Opt. Express 13(19), 7449–7457 (2005). [CrossRef] [PubMed]
  26. A. Davis, J. Izatt, and F. Rothenberg, “Quantitative measurement of blood flow dynamics in embryonic vasculature using spectral Doppler velocimetry,” Anat. Rec. (Hoboken) 292(3), 311–319 (2009). [CrossRef] [PubMed]
  27. M. Kass, A. Witkin, and D. Terzopoulos, “Snakes: Active Contour Models,” Int. J. Comput. Vis. 1(4), 321–331 (1988). [CrossRef]
  28. S. Rugonyi, C. Shaut, A. Liu, K. Thornburg, and R. K. Wang, “Changes in wall motion and blood flow in the outflow tract of chick embryonic hearts observed with optical coherence tomography after outflow tract banding and vitelline-vein ligation,” Phys. Med. Biol. 53(18), 5077–5091 (2008). [CrossRef] [PubMed]
  29. Y. C. Ahn, W. Jung, and Z. Chen, “Quantification of a three-dimensional velocity vector using spectral-domain Doppler optical coherence tomography,” Opt. Lett. 32(11), 1587–1589 (2007). [CrossRef] [PubMed]

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