OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 3 — Oct. 1, 2010
  • pp: 895–901

Differential microscopy for fluorescence-detected nonlinear absorption linear anisotropy based on a staggered two-beam femtosecond Yb:KGW oscillator

Daaf Sandkuijl, Richard Cisek, Arkady Major, and Virginijus Barzda  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 3, pp. 895-901 (2010)
http://dx.doi.org/10.1364/BOE.1.000895


View Full Text Article

Enhanced HTML    Acrobat PDF (2039 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present a new laser system and nonlinear microscope, designed for differential nonlinear microscopy. The microscope features time-correlated single photon counting of multiphoton fluorescence generated by an alternating pulse-train of orthogonally polarized pulses. The generated nonlinear signal is separated using home-built electronics. Results are presented on fluorescence-detected nonlinear absorption linear anisotropy (FDNALA) of chloroplasts in Asparagus Sprengerii Regel and of Congo Red-stained cellulose.

© 2010 OSA

OCIS Codes
(140.3615) Lasers and laser optics : Lasers, ytterbium
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: August 23, 2010
Revised Manuscript: September 9, 2010
Manuscript Accepted: September 10, 2010
Published: September 14, 2010

Citation
Daaf Sandkuijl, Richard Cisek, Arkady Major, and Virginijus Barzda, "Differential microscopy for fluorescence-detected nonlinear absorption linear anisotropy based on a staggered two-beam femtosecond Yb:KGW oscillator," Biomed. Opt. Express 1, 895-901 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-3-895


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. R. Carriles, K. E. Sheetz, E. E. Hoover, J. A. Squier, and V. Barzda, “Simultaneous multifocal, multiphoton, photon counting microscopy,” Opt. Express 16(14), 10364–10371 (2008). [CrossRef] [PubMed]
  2. R. Cisek, L. Spencer, N. Prent, D. Zigmantas, G. S. Espie, and V. Barzda, “Optical microscopy in photosynthesis,” Photosynth. Res. 102(2-3), 111–141 (2009). [CrossRef] [PubMed]
  3. I.-H. Chen, S.-W. Chu, C.-K. Sun, P.-C. Cheng, and B.-L. Lin, “Wavelength dependent damage in biological multi-photon confocal microscopy: A micro-spectroscopic comparison between femtosecond Ti:sapphire and Cr:forsterite laser sources,” Opt. Quantum Electron. 34(12), 1251–1266 (2002). [CrossRef]
  4. A. Major, R. Cisek, and V. Barzda, “Femtosecond Yb:KGd(WO(4))(2) laser oscillator pumped by a high power fiber-coupled diode laser module,” Opt. Express 14(25), 12163–12168 (2006). [CrossRef] [PubMed]
  5. A. Major, V. Barzda, P. A. E. Piunno, S. Musikhin, and U. J. Krull, “An extended cavity diode-pumped femtosecond Yb:KGW laser for applications in optical DNA sensor technology based on fluorescence lifetime measurements,” Opt. Express 14(12), 5285–5294 (2006). [CrossRef] [PubMed]
  6. U. Keller, K. J. Weingarten, F. X. Kartner, D. Kopf, B. Braun, I. D. Jung, R. Fluck, C. Honninger, N. Matuschek, and J. Aus der Au,, “Semiconductor saturable absorber mirrors (SESAM’s) for femtosecond to nanosecond pulse generation in solid-state lasers,” IEEE J. Sel. Top. Quantum Electron. 2(3), 435–453 (1996). [CrossRef]
  7. G. R. Holtom, “Mode-locked Yb:KGW laser longitudinally pumped by polarization-coupled diode bars,” Opt. Lett. 31(18), 2719–2721 (2006). [CrossRef] [PubMed]
  8. G. Steinbach, I. Pomozi, O. Zsiros, A. Pay, G. V. Horvath, and G. Garab, “Imaging fluorescence detected linear dichroism of plant cell walls in laser scanning confocal microscopy,” Cytometry A 73A(3), 202–208 (2008). [CrossRef]
  9. L. Finzi, C. Bustamante, G. Garab, and C.-B. Juang, “Direct observation of large chiral domains in chloroplast thylakoid membranes by differential polarization microscopy,” Proc. Natl. Acad. Sci. U.S.A. 86(22), 8748–8752 (1989). [CrossRef] [PubMed]
  10. P. J. Walla, J. Yom, B. P. Krueger, and G. R. Fleming, “Two-photon excitation spectrum of light-harvesting complex II and fluorescence upconversion after one- and two-photon excitation of the carotenoids,” J. Phys. Chem. B 104(19), 4799–4806 (2000). [CrossRef]
  11. H. Puchtler, F. Sweat, and M. Levine, “On the binding of Congo red by amyloid,” J. Hist. Cyt 10(3), 355–364 (1962).
  12. R. M. Brown, A. C. Millard, and P. J. Campagnola, “Macromolecular structure of cellulose studied by second-harmonic generation imaging microscopy,” Opt. Lett. 28(22), 2207–2209 (2003). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited