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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 3 — Oct. 1, 2010
  • pp: 895–901

Differential microscopy for fluorescence-detected nonlinear absorption linear anisotropy based on a staggered two-beam femtosecond Yb:KGW oscillator

Daaf Sandkuijl, Richard Cisek, Arkady Major, and Virginijus Barzda  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 3, pp. 895-901 (2010)

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We present a new laser system and nonlinear microscope, designed for differential nonlinear microscopy. The microscope features time-correlated single photon counting of multiphoton fluorescence generated by an alternating pulse-train of orthogonally polarized pulses. The generated nonlinear signal is separated using home-built electronics. Results are presented on fluorescence-detected nonlinear absorption linear anisotropy (FDNALA) of chloroplasts in Asparagus Sprengerii Regel and of Congo Red-stained cellulose.

© 2010 OSA

OCIS Codes
(140.3615) Lasers and laser optics : Lasers, ytterbium
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: August 23, 2010
Revised Manuscript: September 9, 2010
Manuscript Accepted: September 10, 2010
Published: September 14, 2010

Daaf Sandkuijl, Richard Cisek, Arkady Major, and Virginijus Barzda, "Differential microscopy for fluorescence-detected nonlinear absorption linear anisotropy based on a staggered two-beam femtosecond Yb:KGW oscillator," Biomed. Opt. Express 1, 895-901 (2010)

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  1. R. Carriles, K. E. Sheetz, E. E. Hoover, J. A. Squier, and V. Barzda, “Simultaneous multifocal, multiphoton, photon counting microscopy,” Opt. Express 16(14), 10364–10371 (2008). [CrossRef] [PubMed]
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