OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 3 — Oct. 1, 2010
  • pp: 911–922

High-resolution fiber optic microscopy with fluorescent contrast enhancement for the identification of axillary lymph node metastases in breast cancer: a pilot study

Kelsey J. Rosbach, Dongsuk Shin, Timothy J. Muldoon, Mohammad A. Quraishi, Lavinia P. Middleton, Kelly K. Hunt, Funda Meric-Bernstam, Tse-Kuan Yu, Rebecca R. Richards-Kortum, and Wei Yang  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 3, pp. 911-922 (2010)
http://dx.doi.org/10.1364/BOE.1.000911


View Full Text Article

Enhanced HTML    Acrobat PDF (35982 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

This prospective pilot study evaluates the potential of high-resolution fiber optic microscopy (HRFM) to identify lymph node metastases in breast cancer patients. 43 lymph nodes were collected from 14 consenting breast cancer patients. Proflavine dye was topically applied to lymph nodes ex vivo to allow visualization of nuclei. 242 images were collected at 105 sites with confirmed histopathologic diagnosis. Quantitative statistical features were calculated from images, assessed with one-way ANOVA, and were used to develop a classification algorithm with the goal of objectively discriminating between normal and metastatic tissue. A classification algorithm using mean image intensity and skewness achieved sensitivity of 79% (27/34) and specificity of 77% (55/71). This study demonstrates the technical feasibility and diagnostic potential of HRFM with fluorescent contrast in the ex vivo evaluation of lymph nodes from breast cancer patients.

© 2010 OSA

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Optics in Cancer Research

History
Original Manuscript: July 30, 2010
Revised Manuscript: September 11, 2010
Manuscript Accepted: September 11, 2010
Published: September 16, 2010

Citation
Kelsey J. Rosbach, Dongsuk Shin, Timothy J. Muldoon, Mohammad A. Quraishi, Lavinia P. Middleton, Kelly K. Hunt, Funda Meric-Bernstam, Tse-Kuan Yu, Rebecca R. Richards-Kortum, and Wei Yang, "High-resolution fiber optic microscopy with fluorescent contrast enhancement for the identification of axillary lymph node metastases in breast cancer: a pilot study," Biomed. Opt. Express 1, 911-922 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-3-911


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. U. S. Cancer Statistics Working Group, “United States Cancer Statistics: 1999–2005 Incidence and Mortality Web-based Report,” Atlanta: U.S. Department of Health and Human Services, Centers for Disease Control and Prevention and National Cancer Institute, 2009, www.cdc.gov/uscs .
  2. M. Duff, A. D. Hill, G. McGreal, S. Walsh, E. W. McDermott, and N. J. O’Higgins, “Prospective evaluation of the morbidity of axillary clearance for breast cancer,” Br. J. Surg. 88(1), 114–117 (2001). [CrossRef] [PubMed]
  3. R. J. Kennedy, J. Bradley, R. W. Parks, and S. J. Kirk, “Prospective evaluation of the morbidity of axillary clearance for breast cancer (Br J Surg 2001; 88: 114-7),” Br. J. Surg. 88(6), 891 (2001). [CrossRef] [PubMed]
  4. W. Kwan, J. Jackson, L. M. Weir, C. Dingee, G. McGregor, and I. A. Olivotto, “Chronic arm morbidity after curative breast cancer treatment: prevalence and impact on quality of life,” J. Clin. Oncol. 20(20), 4242–4248 (2002). [CrossRef] [PubMed]
  5. J. Engel, J. Kerr, A. Schlesinger-Raab, H. Sauer, and D. Hölzel, “Axilla surgery severely affects quality of life: results of a 5-year prospective study in breast cancer patients,” Breast Cancer Res. Treat. 79(1), 47–57 (2003). [CrossRef] [PubMed]
  6. S. A. Norman, A. R. Localio, S. L. Potashnik, H. A. Simoes Torpey, M. J. Kallan, A. L. Weber, L. T. Miller, A. Demichele, and L. J. Solin, “Lymphedema in breast cancer survivors: incidence, degree, time course, treatment, and symptoms,” J. Clin. Oncol. 27(3), 390–397 (2008). [CrossRef] [PubMed]
  7. J. J. Albertini, G. H. Lyman, C. Cox, T. Yeatman, L. Balducci, N. Ku, S. Shivers, C. Berman, K. Wells, D. Rapaport, A. Shons, J. Horton, H. Greenberg, S. Nicosia, R. Clark, A. Cantor, and D. S. Reintgen, “Lymphatic mapping and sentinel node biopsy in the patient with breast cancer,” JAMA 276(22), 1818–1822 (1996). [CrossRef] [PubMed]
  8. D. Krag, D. Weaver, T. Ashikaga, F. Moffat, V. S. Klimberg, C. Shriver, S. Feldman, R. Kusminsky, M. Gadd, J. Kuhn, S. Harlow, P. Beitsch, P. Whitworth, R. Foster, and K. Dowlatshahi, “The sentinel node in breast cancer--a multicenter validation study,” N. Engl. J. Med. 339(14), 941–946 (1998). [CrossRef] [PubMed]
  9. K. M. McMasters, A. E. Giuliano, M. I. Ross, D. S. Reintgen, K. K. Hunt, D. R. Byrd, V. S. Klimberg, P. W. Whitworth, L. C. Tafra, and M. J. Edwards, “Sentinel-lymph-node biopsy for breast cancer--not yet the standard of care,” N. Engl. J. Med. 339(14), 990–995 (1998). [CrossRef] [PubMed]
  10. R. K. Orr, J. L. Hoehn, and N. F. Col, “The learning curve for sentinel node biopsy in breast cancer: practical considerations,” Arch. Surg. 134(7), 764–767 (1999). [CrossRef] [PubMed]
  11. U. Veronesi, G. Paganelli, V. Galimberti, G. Viale, S. Zurrida, M. Bedoni, A. Costa, C. de Cicco, J. G. Geraghty, A. Luini, V. Sacchini, and P. Veronesi, “Sentinel-node biopsy to avoid axillary dissection in breast cancer with clinically negative lymph-nodes,” Lancet 349(9069), 1864–1867 (1997). [CrossRef] [PubMed]
  12. U. Veronesi, G. Paganelli, G. Viale, V. Galimberti, A. Luini, S. Zurrida, C. Robertson, V. Sacchini, P. Veronesi, E. Orvieto, C. De Cicco, M. Intra, G. Tosi, and D. Scarpa, “Sentinel lymph node biopsy and axillary dissection in breast cancer: results in a large series,” J. Natl. Cancer Inst. 91(4), 368–373 (1999). [CrossRef] [PubMed]
  13. A. Lee, S. Krishnamurthy, A. Sahin, W. F. Symmans, K. Hunt, and N. Sneige, “Intraoperative touch imprint of sentinel lymph nodes in breast carcinoma patients,” Cancer 96(4), 225–231 (2002). [CrossRef] [PubMed]
  14. A. F. Gmitro and D. Aziz, “Confocal microscopy through a fiber-optic imaging bundle,” Opt. Lett. 18(8), 565 (1993). [CrossRef] [PubMed]
  15. R. F. Hwang, S. Krishnamurthy, K. K. Hunt, N. Mirza, F. C. Ames, B. Feig, H. M. Kuerer, S. E. Singletary, G. Babiera, F. Meric, J. S. Akins, J. Neely, and M. I. Ross, “Clinicopathologic factors predicting involvement of nonsentinel axillary nodes in women with breast cancer,” Ann. Surg. Oncol. 10(3), 248–254 (2003). [CrossRef] [PubMed]
  16. B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods 2(12), 941–950 (2005). [CrossRef] [PubMed]
  17. L. R. Ferguson and W. A. Denny, “The genetic toxicology of acridines,” Mutat. Res. 258(2), 123–160 (1991). [PubMed]
  18. T. J. Muldoon, M. C. Pierce, D. L. Nida, M. D. Williams, A. Gillenwater, and R. Richards-Kortum, “Subcellular-resolution molecular imaging within living tissue by fiber microendoscopy,” Opt. Express 15(25), 16413–16423 (2007). [CrossRef] [PubMed]
  19. T. J. Muldoon, S. Anandasabapathy, D. Maru, and R. Richards-Kortum, “High-resolution imaging in Barrett’s esophagus: a novel, low-cost endoscopic microscope,” Gastrointest. Endosc. 68(4), 737–744 (2008). [CrossRef] [PubMed]
  20. D. W. Edlow and D. Carter, “Heterotopic epithelium in axillary lymph nodes: report of a case and review of the literature,” Am. J. Clin. Pathol. 59(5), 666–673 (1973). [PubMed]
  21. S. Alvarez, E. Añorbe, P. Alcorta, F. López, I. Alonso, and J. Cortés, “Role of sonography in the diagnosis of axillary lymph node metastases in breast cancer: a systematic review,” AJR Am. J. Roentgenol. 186(5), 1342–1348 (2006). [CrossRef] [PubMed]
  22. M. de Boer, J. A. van Dijck, P. Bult, G. F. Borm, and V. C. Tjan-Heijnen, “Breast cancer prognosis and occult lymph node metastases, isolated tumor cells, and micrometastases,” J. Natl. Cancer Inst. 102(6), 410–425 (2010). [CrossRef] [PubMed]
  23. P. Gimbergues, M. M. Dauplat, X. Durando, C. Abrial, G. Bouedec, M. A. Mouret-Reynier, F. Cachin, F. Kwiatkowski, A. Tchirkov, J. Dauplat, and F. Penault-Llorca, “Intraoperative imprint cytology examination of sentinel lymph nodes after neoadjuvant chemotherapy in breast cancer patients,” Ann. Surg. Oncol. 17(8), 2132–2137 (2010). [CrossRef] [PubMed]
  24. F. Crippa, A. Gerali, A. Alessi, R. Agresti, and E. Bombardieri, “FDG-PET for axillary lymph node staging in primary breast cancer,” Eur. J. Nucl. Med. Mol. Imaging 31(0Suppl 1), S97–S102 (2004). [CrossRef] [PubMed]
  25. M. Pramanik, K. H. Song, M. Swierczewska, D. Green, B. Sitharaman, and L. V. Wang, “In vivo carbon nanotube-enhanced non-invasive photoacoustic mapping of the sentinel lymph node,” Phys. Med. Biol. 54(11), 3291–3301 (2009). [CrossRef] [PubMed]
  26. K. H. Song, C. Kim, K. Maslov, and L. V. Wang, “Noninvasive in vivo spectroscopic nanorod-contrast photoacoustic mapping of sentinel lymph nodes,” Eur. J. Radiol. 70(2), 227–231 (2009). [CrossRef] [PubMed]
  27. J. C. Boughey, J. P. Moriarty, A. C. Degnim, M. S. Gregg, J. S. Egginton, and K. H. Long, “Cost modeling of preoperative axillary ultrasound and fine-needle aspiration to guide surgery for invasive breast cancer,” Ann. Surg. Oncol. 17(4), 953–958 (2010). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4 Fig. 5
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited