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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 3 — Oct. 1, 2010
  • pp: 975–982

Cancer field effects in normal tissues revealed by Raman spectroscopy

Chad A. Lieber, Hubert E. Nethercott, and Mustafa H. Kabeer  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 3, pp. 975-982 (2010)
http://dx.doi.org/10.1364/BOE.1.000975


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Abstract

It has been demonstrated that the presence of cancer results in detectable changes to uninvolved tissues, collectively termed cancer field effects (CFE). In this study, we directly assessed the ability of Raman microspectroscopy to detect CFE via in-vitro study of organotypic tissue rafts approximating human skin. Raman spectra were measured from both epidermis and dermis after transfer of the rafts to dishes containing adherent cultures of either normal human fibroblasts or fibrosarcoma (HT1080) cells. Principal components analyses allowed discrimination between the groups with 86% classification accuracy in the epidermis and 94% in the dermis. These results encourage further study to evaluate the Raman capacity for detecting CFE as a possible tool for noninvasive screening for tumor presence.

© 2010 OSA

OCIS Codes
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Spectroscopic Diagnostics

History
Original Manuscript: August 30, 2010
Revised Manuscript: September 13, 2010
Manuscript Accepted: September 18, 2010
Published: September 20, 2010

Citation
Chad A. Lieber, Hubert E. Nethercott, and Mustafa H. Kabeer, "Cancer field effects in normal tissues revealed by Raman spectroscopy," Biomed. Opt. Express 1, 975-982 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-3-975


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