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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 4 — Nov. 1, 2010
  • pp: 1127–1137

Non-invasive in vivo measurement of the tear film using spatial autocorrelation in a live mammal model

Kaveh Azartash, Chyong-jy Nein Shy, Kevin Flynn, James V. Jester, and Enrico Gratton  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 4, pp. 1127-1137 (2010)
http://dx.doi.org/10.1364/BOE.1.001127


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Abstract

Tear film stability and its interaction with the corneal surface play an important role in maintaining ocular surface integrity and quality of vision. We present a non-invasive technique to quantify the pre-corneal tear film thickness. A cMOS camera is used to record the interference pattern produced by the reflections from multiple layers of the tear film Principles of spatial autocorrelation are applied to extract the frequency of the periodic patterns in the images. A mathematical model is developed to obtain the thickness of the tear film from the spatial autocorrelation image. The technique is validated using micro-fabricated thin parylene films. We obtained repeatable and precise measurement on a live rabbit model (N = 6). We obtained an average value of 10.2µm and standard deviation of, SD = 0.3 (N = 4). We measured one rabbit infected with HSV-1 virus that had a baseline tear film thickness of 4.7µm.

© 2010 OSA

OCIS Codes
(170.4460) Medical optics and biotechnology : Ophthalmic optics and devices
(170.4470) Medical optics and biotechnology : Ophthalmology

ToC Category:
Ophthalmology Applications

History
Original Manuscript: July 19, 2010
Revised Manuscript: October 3, 2010
Manuscript Accepted: October 7, 2010
Published: October 8, 2010

Citation
Kaveh Azartash, Chyong-jy Nein Shy, Kevin Flynn, James V. Jester, and Enrico Gratton, "Non-invasive in vivo measurement of the tear film using spatial autocorrelation in a live mammal model," Biomed. Opt. Express 1, 1127-1137 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-4-1127


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