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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 4 — Nov. 1, 2010
  • pp: 1159–1172

Development of a versatile two-photon endoscope for biological imaging

Youbo Zhao, Hiroshi Nakamura, and Robert J. Gordon  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 4, pp. 1159-1172 (2010)

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We describe a versatile, catheter-type two-photon probe, designed for in vivo and ex vivo imaging of the aqueous outflow pathway in the eye. The device consists of a silica double cladding fiber used for laser delivery and fluorescence collection, a spiral fiber scanner driven by a miniature piezoelectric tube, and an assembly of three micro-size doublet achromatic lenses used for focusing the laser and collecting the two-photon excitation signal. All the components have a maximum diameter of 2 mm and are enclosed in a length of 12-gauge stainless steel hypodermic tubing having an outer diameter of 2.8 mm. The lateral and axial resolutions of the probe are measured to be 1.5 μm and 9.2 μm, respectively. Different lens configurations and fibers are evaluated by comparing their spatial resolutions and fluorescence signal collection efficiencies. Doublet achromatic lenses and a double cladding fiber with a high inner cladding numerical aperture are found to produce a high signal collection efficiency, which is essential for imaging live tissues. Simple methods for reducing image distortions are demonstrated. Images of human trabecular meshwork tissue are successfully obtained with this miniature two-photon microscope.

© 2010 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.2350) Imaging systems : Fiber optics imaging
(170.0110) Medical optics and biotechnology : Imaging systems
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Endoscopes, Catheters and Micro-Optics

Original Manuscript: July 23, 2010
Revised Manuscript: September 21, 2010
Manuscript Accepted: October 3, 2010
Published: October 13, 2010

Youbo Zhao, Hiroshi Nakamura, and Robert J. Gordon, "Development of a versatile two-photon endoscope for biological imaging," Biomed. Opt. Express 1, 1159-1172 (2010)

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