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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 4 — Nov. 1, 2010
  • pp: 1196–1208

Depth-resolved measurement of mucosal microvascular blood content using low-coherence enhanced backscattering spectroscopy

Andrew J. Radosevich, Vladimir M. Turzhitsky, Nikhil N. Mutyal, Jeremy D. Rogers, Valentina Stoyneva, Ashish Kumar Tiwari, Mart De La Cruz, Dhananjay P. Kunte, Ramesh K. Wali, Hemant K. Roy, and Vadim Backman  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 4, pp. 1196-1208 (2010)
http://dx.doi.org/10.1364/BOE.1.001196


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Abstract

Low-coherence enhanced backscattering (LEBS) spectroscopy is a light scattering technique which uses partial spatial coherence broadband illumination to interrogate the optical properties at sub-diffusion length scales. In this work, we present a post-processing technique which isolates the hemoglobin concentration at different depths within a sample using a single spectroscopic LEBS measurement with a fixed spatial coherence of illumination. We verify the method with scattering (spectralon reflectance standard and polystyrene microspheres) and absorbing (hemoglobin) phantoms. We then demonstrate the relevance of this method for quantifying hemoglobin content as a function of depth within biological tissue using the azoxymethane treated animal model of colorectal cancer.

© 2010 OSA

OCIS Codes
(030.0030) Coherence and statistical optics : Coherence and statistical optics
(290.1350) Scattering : Backscattering
(300.6550) Spectroscopy : Spectroscopy, visible

ToC Category:
Spectroscopic Diagnostics

History
Original Manuscript: September 7, 2010
Revised Manuscript: October 10, 2010
Manuscript Accepted: October 17, 2010
Published: October 20, 2010

Citation
Andrew J. Radosevich, Vladimir M. Turzhitsky, Nikhil N. Mutyal, Jeremy D. Rogers, Valentina Stoyneva, Ashish Kumar Tiwari, Mart De La Cruz, Dhananjay P. Kunte, Ramesh K. Wali, Hemant K. Roy, and Vadim Backman, "Depth-resolved measurement of mucosal microvascular blood content using 
low-coherence enhanced backscattering spectroscopy," Biomed. Opt. Express 1, 1196-1208 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-4-1196


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