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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 4 — Nov. 1, 2010
  • pp: 1217–1224

Measuring micro-interactions between coagulating red blood cells using optical tweezers

Bor-Wen Yang and Zhe Li  »View Author Affiliations

Biomedical Optics Express, Vol. 1, Issue 4, pp. 1217-1224 (2010)

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Agents that alter the dynamics of hemostasis form an important part in management of conditions such as atherosclerosis, cerebrovascular disease, and bleeding diatheses. In this study, we explored the effects of heparin and tranexamic acid on the efficiency of blood coagulation. Using optical tweezers, we evaluated the pN-range micro-interaction between coagulating red blood cells (RBCs) by measuring the minimum power required to trap them. By observing the mobility of RBCs and the intensity of cellular interactions, we found that the coagulation process can be separated into three phases. The effects of heparin and tranexamic acid were examined by observing variations in cellular interaction during the coagulation phases. Heparin attenuated the interaction between RBCs and prolonged the first phase whereas the samples containing tranexamic acid bypassed the first two phases and immediately proceeded to the final one.

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OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Traps, Manipulation, and Tracking

Original Manuscript: August 16, 2010
Revised Manuscript: September 26, 2010
Manuscript Accepted: October 19, 2010
Published: October 22, 2010

Bor-Wen Yang and Zhe Li, "Measuring micro-interactions between coagulating red blood cells using optical tweezers," Biomed. Opt. Express 1, 1217-1224 (2010)

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