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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 1, Iss. 5 — Dec. 1, 2010
  • pp: 1408–1431

High-throughput FCS using an LCOS spatial light modulator and an 8 × 1 SPAD array

Ryan A. Colyer, Giuseppe Scalia, Ivan Rech, Angelo Gulinatti, Massimo Ghioni, Sergio Cova, Shimon Weiss, and Xavier Michalet  »View Author Affiliations


Biomedical Optics Express, Vol. 1, Issue 5, pp. 1408-1431 (2010)
http://dx.doi.org/10.1364/BOE.1.001408


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Abstract

We present a novel approach to high-throughput Fluorescence Correlation Spectroscopy (FCS) which enables us to obtain one order of magnitude improvement in acquisition time. Our approach utilizes a liquid crystal on silicon spatial light modulator to generate dynamically adjustable focal spots, and uses an eight-pixel monolithic single-photon avalanche photodiode array. We demonstrate the capabilities of this system by showing FCS of Rhodamine 6G under various viscosities, and by showing that, with proper calibration of each detection channel, one order of magnitude improvement in acquisition speed is obtained. More generally, our approach will allow higher throughput single-molecule studies to be performed.

© 2010 OSA

OCIS Codes
(040.1240) Detectors : Arrays
(040.6070) Detectors : Solid state detectors
(180.2520) Microscopy : Fluorescence microscopy
(230.6120) Optical devices : Spatial light modulators
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Spectroscopic Diagnostics

History
Original Manuscript: August 18, 2010
Revised Manuscript: October 14, 2010
Manuscript Accepted: November 8, 2010
Published: November 15, 2010

Virtual Issues
November 16, 2010 Spotlight on Optics

Citation
Ryan A. Colyer, Giuseppe Scalia, Ivan Rech, Angelo Gulinatti, Massimo Ghioni, Sergio Cova, Shimon Weiss, and Xavier Michalet, "High-throughput FCS using an LCOS spatial light modulator and an 8 × 1 SPAD array," Biomed. Opt. Express 1, 1408-1431 (2010)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-5-1408


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