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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 1 — Jan. 1, 2011
  • pp: 139–148

Images of photoreceptors in living primate eyes using adaptive optics two-photon ophthalmoscopy

Jennifer J. Hunter, Benjamin Masella, Alfredo Dubra, Robin Sharma, Lu Yin, William H. Merigan, Grazyna Palczewska, Krzysztof Palczewski, and David R. Williams  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 1, pp. 139-148 (2011)
http://dx.doi.org/10.1364/BOE.2.000139


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Abstract

In vivo two-photon imaging through the pupil of the primate eye has the potential to become a useful tool for functional imaging of the retina. Two-photon excited fluorescence images of the macaque cone mosaic were obtained using a fluorescence adaptive optics scanning laser ophthalmoscope, overcoming the challenges of a low numerical aperture, imperfect optics of the eye, high required light levels, and eye motion. Although the specific fluorophores are as yet unknown, strong in vivo intrinsic fluorescence allowed images of the cone mosaic. Imaging intact ex vivo retina revealed that the strongest two-photon excited fluorescence signal comes from the cone inner segments. The fluorescence response increased following light stimulation, which could provide a functional measure of the effects of light on photoreceptors.

© 2010 OSA

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(330.4460) Vision, color, and visual optics : Ophthalmic optics and devices
(330.5310) Vision, color, and visual optics : Vision - photoreceptors
(330.7327) Vision, color, and visual optics : Visual optics, ophthalmic instrumentation

ToC Category:
Ophthalmology Applications

History
Original Manuscript: November 8, 2010
Revised Manuscript: December 14, 2010
Manuscript Accepted: December 14, 2010
Published: December 17, 2010

Citation
Jennifer J. Hunter, Benjamin Masella, Alfredo Dubra, Robin Sharma, Lu Yin, William H. Merigan, Grazyna Palczewska, Krzysztof Palczewski, and David R. Williams, "Images of photoreceptors in living primate eyes using adaptive optics two-photon ophthalmoscopy," Biomed. Opt. Express 2, 139-148 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-1-139


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