OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 1 — Jan. 1, 2011
  • pp: 185–193

A cylindrical zoom lens unit for adjustable optical sectioning in light sheet microscopy

Jörg G. Ritter, Jan-Hendrik Spille, Tim Kaminski, and Ulrich Kubitscheck  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 1, pp. 185-193 (2011)
http://dx.doi.org/10.1364/BOE.2.000185


View Full Text Article

Enhanced HTML    Acrobat PDF (1171 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Light sheet microscopy became a powerful tool in life sciences. Often, however, the sheet geometry is fixed, whereas it would be advantageous to adjust the sheet geometry to specimens of different dimensions. Therefore we developed an afocal cylindrical zoom lens system comprising only 5 lenses and a total system length of less than 160 mm. Two movable optical elements were directly coupled, so that the zoom factor could be adjusted from 1x to 6.3x by a single motor. Using two different illumination objectives we achieved a light sheet thickness ranging from 2.4 µm to 36 µm corresponding to lateral fields of 54 µm to 12.3 mm, respectively. Polytene chromosomes of salivary gland cell nuclei of C.tentans larvae were imaged in vivo to demonstrate the advantages in image contrast by imaging with different light sheet dimensions.

© OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.0180) Microscopy : Microscopy
(170.2945) Medical optics and biotechnology : Illumination design

ToC Category:
Microscopy

History
Original Manuscript: October 26, 2010
Manuscript Accepted: December 15, 2010
Published: December 20, 2010

Citation
Jörg G. Ritter, Jan-Hendrik Spille, Tim Kaminski, and Ulrich Kubitscheck, "A cylindrical zoom lens unit for adjustable optical sectioning in light sheet microscopy," Biomed. Opt. Express 2, 185-193 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-1-185


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. J. Vermot, S. E. Fraser, and M. Liebling, “Fast fluorescence microscopy for imaging the dynamics of embryonic development,” HFSP J 2(3), 143–155 (2008). [CrossRef] [PubMed]
  2. E. G. Reynaud, U. Krzic, K. Greger, and E. H. Stelzer, “Light sheet-based fluorescence microscopy: more dimensions, more photons, and less photodamage,” HFSP J 2(5), 266–275 (2008). [CrossRef] [PubMed]
  3. J. Huisken and D. Y. Stainier, “Selective plane illumination microscopy techniques in developmental biology,” Development 136(12), 1963–1975 (2009). [CrossRef] [PubMed]
  4. N. Jährling, K. Becker, C. Schönbauer, F. Schnorrer, and H. U. Dodt, “Three-dimensional reconstruction and segmentation of intact Drosophila by ultramicroscopy,” Front Syst Neurosci 4, 1 (2010). [PubMed]
  5. P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7(8), 637–642 (2010). [CrossRef] [PubMed]
  6. J. A. Buytaert and J. J. Dirckx, “Tomographic imaging of macroscopic biomedical objects in high resolution and three dimensions using orthogonal-plane fluorescence optical sectioning,” Appl. Opt. 48(5), 941–948 (2009). [CrossRef] [PubMed]
  7. J. G. Ritter, R. Veith, A. Veenendaal, J. P. Siebrasse, and U. Kubitscheck, “Light sheet microscopy for single molecule tracking in living tissue,” PLoS ONE 5(7), e11639 (2010). [CrossRef] [PubMed]
  8. S. Preibisch, S. Saalfeld, J. Schindelin, and P. Tomancak, “Software for bead-based registration of selective plane illumination microscopy data,” Nat. Methods 7(6), 418–419 (2010). [CrossRef] [PubMed]
  9. E. G. Reynaud and P. Tomancak, “Meeting report: first light sheet based fluorescence microscopy workshop,” Biotechnol. J. 5(8), 798–804 (2010). [CrossRef] [PubMed]
  10. T. Wohland, X. Shi, J. Sankaran, and E. H. Stelzer, “Single plane illumination fluorescence correlation spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environments,” Opt. Express 18(10), 10627–10641 (2010). [CrossRef] [PubMed]
  11. S. Saghafi, K. Becker, N. Jährling, M. Richter, E. R. Kramer, and H. U. Dodt, “Image enhancement in ultramicroscopy by improved laser light sheets,” J Biophotonics 3(10-11), 686–695 (2010). [CrossRef] [PubMed]
  12. B. E. A. Saleh, and M. C. Teich, Fundamentals of Photonics (John Wiley & Sons, 2007).
  13. H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
  14. J Winterot, P. Huettel, “Microscope with an afocal zoom system,” EP1544653A1 (2005).
  15. L. Wieslander, “The Balbiani ring multigene family: coding repetitive sequences and evolution of a tissue-specific cell function,” Prog. Nucleic Acid Res. Mol. Biol. 48, 275–313 (1994). [CrossRef] [PubMed]
  16. E. Wurtz-T, G. Kiseleva, A. Nacheva, A. Alzhanova-Ericcson, Rosén, and B. Daneholt, “Identification of two RNA-binding proteins in Balbiani ring premessenger ribonucleoprotein granules and presence of these proteins in specific subsets of heterogeneous nuclear ribonucleoprotein particles,” Mol. Cell. Biol. 16(4), 1425–1435 (1996). [PubMed]
  17. J. Huisken and D. Y. Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett. 32(17), 2608–2610 (2007). [CrossRef] [PubMed]
  18. B. Münch, P. Trtik, F. Marone, and M. Stampanoni, “Stripe and ring artifact removal with combined wavelet--Fourier filtering,” Opt. Express 17(10), 8567–8591 (2009). [CrossRef] [PubMed]
  19. S. Kalchmair, N. Jährling, K. Becker, and H. U. Dodt, “Image contrast enhancement in confocal ultramicroscopy,” Opt. Lett. 35(1), 79–81 (2010). [CrossRef] [PubMed]
  20. P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008). [CrossRef] [PubMed]
  21. X. Wang, T. Wohland, and V. Korzh, “Developing in vivo biophysics by fishing for single molecules,” Dev. Biol. 347(1), 1–8 (2010). [CrossRef] [PubMed]
  22. J. G. Ritter, R. Veith, J. P. Siebrasse, and U. Kubitscheck, “High-contrast single-particle tracking by selective focal plane illumination microscopy,” Opt. Express 16(10), 7142–7152 (2008). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited