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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 1 — Jan. 1, 2011
  • pp: 80–88

High speed multiphoton axial scanning through an optical fiber in a remotely scanned temporal focusing setup

Adam Straub, Michael E. Durst, and Chris Xu  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 1, pp. 80-88 (2011)
http://dx.doi.org/10.1364/BOE.2.000080


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Abstract

Simultaneous spatial and temporal focusing is used to acquire high speed (200Hz), chemically specific axial scans of mouse skin through a single-mode fiber. The temporal focus is remotely scanned by modulating the group delay dispersion (GDD) at the proximal end of the fiber. No moving parts or electronics are required at the distal end. A novel GDD modulation technique is implemented using a piezo bimorph mirror in a folded grating pair to achieve a large GDD tuning range at high speed.

© 2010 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.5810) Microscopy : Scanning microscopy
(230.2035) Optical devices : Dispersion compensation devices

ToC Category:
Microscopy

History
Original Manuscript: October 20, 2010
Revised Manuscript: November 7, 2010
Manuscript Accepted: December 2, 2010
Published: December 6, 2010

Citation
Adam Straub, Michael E. Durst, and Chris Xu, "High speed multiphoton axial scanning through an optical fiber in a remotely scanned temporal focusing setup," Biomed. Opt. Express 2, 80-88 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-1-80


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References

  1. Y. Wu, P. Xi, J. Qu, T. H. Cheung, and M. Y. Yu, “Depth-resolved fluorescence spectroscopy of normal and dysplastic cervical tissue,” Opt. Express 13(2), 382–388 (2005). [CrossRef] [PubMed]
  2. M. C. Skala, J. M. Squirrell, K. M. Vrotsos, J. C. Eickhoff, A. Gendron-Fitzpatrick, K. W. Eliceiri, and N. Ramanujam, “Multiphoton microscopy of endogenous fluorescence differentiates normal, precancerous, and cancerous squamous epithelial tissues,” Cancer Res. 65(4), 1180–1186 (2005). [CrossRef] [PubMed]
  3. W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Natl. Acad. Sci. U.S.A. 100(12), 7075–7080 (2003). [CrossRef] [PubMed]
  4. R. Kiesslich, J. Burg, M. Vieth, J. Gnaendiger, M. Enders, P. Delaney, A. Polglase, W. McLaren, D. Janell, S. Thomas, B. Nafe, P. R. Galle, and M. F. Neurath, “Confocal laser endoscopy for diagnosing intraepithelial neoplasias and colorectal cancer in vivo,” Gastroenterology 127(3), 706–713 (2004). [CrossRef] [PubMed]
  5. B. A. Flusberg, J. C. Jung, E. D. Cocker, E. P. Anderson, and M. J. Schnitzer, “In vivo brain imaging using a portable 3.9 gram two-photon fluorescence microendoscope,” Opt. Lett. 30(17), 2272–2274 (2005). [CrossRef] [PubMed]
  6. L. Fu, A. Jain, H. Xie, C. Cranfield, and M. Gu, “Nonlinear optical endoscopy based on a double-clad photonic crystal fiber and a MEMS mirror,” Opt. Express 14(3), 1027–1032 (2006). [CrossRef] [PubMed]
  7. F. Helmchen, M. S. Fee, D. W. Tank, and W. Denk, “A miniature head-mounted two-photon microscope. high-resolution brain imaging in freely moving animals,” Neuron 31(6), 903–912 (2001). [CrossRef] [PubMed]
  8. E. J. Botcherby, R. Juskaitis, M. J. Booth, and T. Wilson, “Aberration-free optical refocusing in high numerical aperture microscopy,” Opt. Lett. 32(14), 2007–2009 (2007). [CrossRef] [PubMed]
  9. V. Nikolenko, B. O. Watson, R. Araya, A. Woodruff, D. S. Peterka, and R. Yuste, “SLM microscopy: scanless two-photon imaging and photostimulation using spatial light modulators,” Front Neural Circuits 2, 5 (2008). [CrossRef] [PubMed]
  10. G. J. Tearney, M. E. Brezinski, B. E. Bouma, S. A. Boppart, C. Pitris, J. F. Southern, and J. G. Fujimoto, “In vivo endoscopic optical biopsy with optical coherence tomography,” Science 276(5321), 2037–2039 (1997). [CrossRef] [PubMed]
  11. G. Zhu, J. van Howe, M. Durst, W. Zipfel, and C. Xu, “Simultaneous spatial and temporal focusing of femtosecond pulses,” Opt. Express 13(6), 2153–2159 (2005). [CrossRef] [PubMed]
  12. D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express 13(5), 1468–1476 (2005). [CrossRef] [PubMed]
  13. M. E. Durst, G. Zhu, and C. Xu, “Simultaneous spatial and temporal focusing for axial scanning,” Opt. Express 14(25), 12243–12254 (2006). [CrossRef] [PubMed]
  14. R. Du, K. Bi, S. Zeng, D. Li, S. Xue, and Q. Luo, “Analysis of fast axial scanning scheme using temporal focusing with acousto-optic deflectors,” J. Mod. Opt. 56, 99–102 (2008).
  15. R. L. Fork, O. E. Martinez, and J. P. Gordon, “Negative dispersion using pairs of prisms,” Opt. Lett. 9(5), 150–152 (1984). [CrossRef] [PubMed]
  16. O. Martinez, “3000 times grating compressor with positive group-velocity dispersion – application to fiber compensation in 1.3–1.6 Mu-M region,” IEEE J. Quantum Electron. 23(1), 59–64 (1987). [CrossRef]
  17. P. Tournois, “Acousto-optic programmable dispersive filter for adaptive compensation of group delay time dispersion in laser systems,” Opt. Commun. 140(4-6), 245–249 (1997). [CrossRef]
  18. A. M. Weiner, D. E. Leaird, J. S. Patel, and J. R. Wullert, “Programmable femtosecond pulse shaping by use of a multielement liquid-crystal phase modulator,” Opt. Lett. 15(6), 326–328 (1990). [CrossRef] [PubMed]
  19. E. Zeek, K. Maginnis, S. Backus, U. Russek, M. Murnane, G. Mourou, H. Kapteyn, and G. Vdovin, “Pulse compression by use of deformable mirrors,” Opt. Lett. 24(7), 493–495 (1999). [CrossRef] [PubMed]
  20. C. Radzewicz, P. Wasylczyk, W. Wasilewski, and J. S. Krasiński, “Piezo-driven deformable mirror for femtosecond pulse shaping,” Opt. Lett. 29(2), 177–179 (2004). [CrossRef] [PubMed]
  21. Q. Li, M. Lovell, J. Mei, and W. Clark, “A study of displacement distribution in a piezoelectric heterogeneous bimorph,” J. Mech. Des. 126(4), 757–762 (2004). [CrossRef]
  22. M. Steel, F. Harrison, and P. Harper, “The piezoelectric bimorph: An experimental and theoretical study of its quasistatic response,” J. Phys. D Appl. Phys. 11(6), 979–989 (1978). [CrossRef]
  23. P. Wnuk, C. Radzewicz, and J. Krasiński, “Bimorph piezo deformable mirror for femtosecond pulse shaping,” Opt. Express 13(11), 4154–4159 (2005). [CrossRef] [PubMed]
  24. “Introduction to piezo transducers” (Piezo Systems, Inc., 2007), http://www.piezo.com/tech2intropiezotrans.html , accessed June 24, 2009.
  25. J. A. Palero, H. S. de Bruijn, A. van der Ploeg van den Heuvel, H. J. Sterenborg, and H. C. Gerritsen, “Spectrally resolved multiphoton imaging of in vivo and excised mouse skin tissues,” Biophys. J. 93(3), 992–1007 (2007). [CrossRef] [PubMed]
  26. S. P. Gross, “Application of optical traps in vivo,” Methods Enzymol. 361, 162–174 (2003). [CrossRef] [PubMed]
  27. K. C. Neuman and S. M. Block, “Optical trapping,” Rev. Sci. Instrum. 75(9), 2787–2809 (2004). [CrossRef] [PubMed]

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