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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 10 — Oct. 1, 2011
  • pp: 2770–2783

High speed spectral domain optical coherence tomography for retinal imaging at 500,000 A‑lines per second

Lin An, Peng Li, Tueng T. Shen, and Ruikang Wang  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 10, pp. 2770-2783 (2011)
http://dx.doi.org/10.1364/BOE.2.002770


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Abstract

We present a new development of ultrahigh speed spectral domain optical coherence tomography (SDOCT) for human retinal imaging at 850 nm central wavelength by employing two high-speed line scan CMOS cameras, each running at 250 kHz. Through precisely controlling the recording and reading time periods of the two cameras, the SDOCT system realizes an imaging speed at 500,000 A-lines per second, while maintaining both high axial resolution (~8 μm) and acceptable depth ranging (~2.5 mm). With this system, we propose two scanning protocols for human retinal imaging. The first is aimed to achieve isotropic dense sampling and fast scanning speed, enabling a 3D imaging within 0.72 sec for a region covering 4x4 mm2. In this case, the B-frame rate is 700 Hz and the isotropic dense sampling is 500 A-lines along both the fast and slow axes. This scanning protocol minimizes the motion artifacts, thus making it possible to perform two directional averaging so that the signal to noise ratio of the system is enhanced while the degradation of its resolution is minimized. The second protocol is designed to scan the retina in a large field of view, in which 1200 A-lines are captured along both the fast and slow axes, covering 10 mm2, to provide overall information about the retinal status. Because of relatively long imaging time (4 seconds for a 3D scan), the motion artifact is inevitable, making it difficult to interpret the 3D data set, particularly in a way of depth-resolved en-face fundus images. To mitigate this difficulty, we propose to use the relatively high reflecting retinal pigmented epithelium layer as the reference to flatten the original 3D data set along both the fast and slow axes. We show that the proposed system delivers superb performance for human retina imaging.

© 2011 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4460) Medical optics and biotechnology : Ophthalmic optics and devices
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Optical Coherence Tomography

History
Original Manuscript: August 8, 2011
Revised Manuscript: September 6, 2011
Manuscript Accepted: September 6, 2011
Published: September 12, 2011

Citation
Lin An, Peng Li, Tueng T. Shen, and Ruikang Wang, "High speed spectral domain optical coherence tomography for retinal imaging at 500,000 A‑lines per second," Biomed. Opt. Express 2, 2770-2783 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-10-2770


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