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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 10 — Oct. 1, 2011
  • pp: 2888–2896

Fast macro-scale transmission imaging of microvascular networks using KESM

David Mayerich, Jaerock Kwon, Chul Sung, Louise Abbott, John Keyser, and Yoonsuck Choe  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 10, pp. 2888-2896 (2011)

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Accurate microvascular morphometric information has significant implications in several fields, including the quantification of angiogenesis in cancer research, understanding the immune response for neural prosthetics, and predicting the nature of blood flow as it relates to stroke. We report imaging of the whole mouse brain microvascular system at resolutions sufficient to perform accurate morphometry. Imaging was performed using Knife-Edge Scanning Microscopy (KESM) and is the first example of this technique that can be directly applied to clinical research. We are able to achieve ≈ 0.7μm resolution laterally with 1μm depth resolution using serial sectioning. No alignment was necessary and contrast was sufficient to allow segmentation and measurement of vessels.

© 2011 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.1020) Medical optics and biotechnology : Ablation of tissue
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.2945) Medical optics and biotechnology : Illumination design

ToC Category:

Original Manuscript: July 19, 2011
Revised Manuscript: August 29, 2011
Manuscript Accepted: September 19, 2011
Published: September 29, 2011

David Mayerich, Jaerock Kwon, Chul Sung, Louise Abbott, John Keyser, and Yoonsuck Choe, "Fast macro-scale transmission imaging of microvascular networks using KESM," Biomed. Opt. Express 2, 2888-2896 (2011)

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