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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 10 — Oct. 1, 2011
  • pp: 2897–2904

In vivo and in situ cellular imaging full-field optical coherence tomography with a rigid endoscopic probe

Anne Latrive and A. Claude Boccara  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 10, pp. 2897-2904 (2011)

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Full-field OCT has proved to be a powerful high-resolution cellular imaging tool for biological tissues. However the standard bulk full-field OCT setup does not match the size requirements for most in situ and in vivo imaging applications. We adapted its principle into a rigid needle-like probe using two coupled interferometers and incoherent illumination: an external processing interferometer is used for in-depth scanning, while a distal common-path interferometer at the tip of the probe collects light backscattered from the tissue. Our experimental setup achieves an axial and transversal resolution in tissue of 1.8 µm and 3.5 µm respectively, for a sensitivity of −80 dB. We present ex vivo images of human breast tissue, and in vivo images of different areas of human skin, which reveal cellular-level structures.

© 2011 OSA

OCIS Codes
(110.2760) Imaging systems : Gradient-index lenses
(110.4500) Imaging systems : Optical coherence tomography
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy

ToC Category:
Optical Coherence Tomography

Original Manuscript: July 11, 2011
Manuscript Accepted: July 23, 2011
Published: September 29, 2011

Anne Latrive and A. Claude Boccara, "In vivo and in situ cellular imaging full-field optical coherence tomography with a rigid endoscopic probe," Biomed. Opt. Express 2, 2897-2904 (2011)

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