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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 11 — Nov. 1, 2011
  • pp: 2950–2960

Femtosecond infrared intrastromal ablation and backscattering-mode adaptive-optics multiphoton microscopy in chicken corneas

Emilio J. Gualda, Javier R. Vázquez de Aldana, M. Carmen Martínez-García, Pablo Moreno, Juan Hernández-Toro, Luis Roso, Pablo Artal, and Juan M. Bueno  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 11, pp. 2950-2960 (2011)

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The performance of femtosecond (fs) laser intrastromal ablation was evaluated with backscattering-mode adaptive-optics multiphoton microscopy in ex vivo chicken corneas. The pulse energy of the fs source used for ablation was set to generate two different ablation patterns within the corneal stroma at a certain depth. Intrastromal patterns were imaged with a custom adaptive-optics multiphoton microscope to determine the accuracy of the procedure and verify the outcomes. This study demonstrates the potential of using fs pulses as surgical and monitoring techniques to systematically investigate intratissue ablation. Further refinement of the experimental system by combining both functions into a single fs laser system would be the basis to establish new techniques capable of monitoring corneal surgery without labeling in real-time. Since the backscattering configuration has also been optimized, future in vivo implementations would also be of interest in clinical environments involving corneal ablation procedures.

© 2011 OSA

OCIS Codes
(170.1020) Medical optics and biotechnology : Ablation of tissue
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4470) Medical optics and biotechnology : Ophthalmology
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Optical Therapies and Photomodificaton

Original Manuscript: July 1, 2011
Revised Manuscript: September 27, 2011
Manuscript Accepted: September 27, 2011
Published: October 3, 2011

Virtual Issues
Advances in Optics for Biotechnology, Medicine, and Surgery (2011) Biomedical Optics Express

Emilio J. Gualda, Javier R. Vázquez de Aldana, M. Carmen Martínez-García, Pablo Moreno, Juan Hernández-Toro, Luis Roso, Pablo Artal, and Juan M. Bueno, "Femtosecond infrared intrastromal ablation and backscattering-mode adaptive-optics multiphoton microscopy in chicken corneas," Biomed. Opt. Express 2, 2950-2960 (2011)

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