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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 11 — Nov. 1, 2011
  • pp: 2982–2994

3D imaging of biofilms on implants by detection of scattered light with a scanning laser optical tomograph

Marko Heidrich, Mark P. Kühnel, Manuela Kellner, Raoul-Amadeus Lorbeer, Tineke Lange, Andreas Winkel, Meike Stiesch, Heiko Meyer, and Alexander Heisterkamp  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 11, pp. 2982-2994 (2011)
http://dx.doi.org/10.1364/BOE.2.002982


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Abstract

Biofilms – communities of microorganisms attached to surfaces – are a constant threat for long-term success in modern implantology. The application of laser scanning microscopy (LSM) has increased the knowledge about microscopic properties of biofilms, whereas a 3D imaging technique for the large scale visualization of bacterial growth and migration on curved and non-transparent surfaces is not realized so far.Towards this goal, we built a scanning laser optical tomography (SLOT) setup detecting scattered laser light to image biofilm on dental implant surfaces. SLOT enables the visualization of living biofilms in 3D by detecting the wavelength-dependent absorption of non-fluorescent stains like e.g. reduced triphenyltetrazolium chloride (TTC) accumulated within metabolically active bacterial cells. Thus, the presented system allows the large scale investigation of vital biofilm structure and in vitro development on cylindrical and non-transparent objects without the need for fluorescent vital staining. We suggest SLOT to be a valuable tool for the structural and volumetric investigation of biofilm formation on implants with sizes up to several millimeters.

© 2011 OSA

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(180.0180) Microscopy : Microscopy
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(290.0290) Scattering : Scattering
(110.6955) Imaging systems : Tomographic imaging

ToC Category:
Microscopy

History
Original Manuscript: August 8, 2011
Revised Manuscript: September 2, 2011
Manuscript Accepted: September 2, 2011
Published: October 3, 2011

Citation
Marko Heidrich, Mark P. Kühnel, Manuela Kellner, Raoul-Amadeus Lorbeer, Tineke Lange, Andreas Winkel, Meike Stiesch, Heiko Meyer, and Alexander Heisterkamp, "3D imaging of biofilms on implants by detection of scattered light with a scanning laser optical tomograph," Biomed. Opt. Express 2, 2982-2994 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-11-2982


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