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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 11 — Nov. 1, 2011
  • pp: 3010–3020

Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging

Ginni Grover, Sean Quirin, Callie Fiedler, and Rafael Piestun  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 11, pp. 3010-3020 (2011)
http://dx.doi.org/10.1364/BOE.2.003010


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Abstract

We present a double-helix point spread function (DH-PSF) based three-dimensional (3D) microscope with efficient photon collection using a phase mask fabricated by gray-level lithography. The system using the phase mask more than doubles the efficiency of current liquid crystal spatial light modulator implementations. We demonstrate the phase mask DH-PSF microscope for 3D photo-activation localization microscopy (PM-DH-PALM) over an extended axial range.

© 2011 OSA

OCIS Codes
(050.1970) Diffraction and gratings : Diffractive optics
(110.4850) Imaging systems : Optical transfer functions
(180.6900) Microscopy : Three-dimensional microscopy
(220.4000) Optical design and fabrication : Microstructure fabrication

ToC Category:
Microscopy

History
Original Manuscript: June 28, 2011
Revised Manuscript: September 16, 2011
Manuscript Accepted: October 1, 2011
Published: October 5, 2011

Citation
Ginni Grover, Sean Quirin, Callie Fiedler, and Rafael Piestun, "Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging," Biomed. Opt. Express 2, 3010-3020 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-11-3010


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