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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 11 — Nov. 1, 2011
  • pp: 3021–3036

Imaging workflow and calibration for CT-guided time-domain fluorescence tomography

Kenneth M. Tichauer, Robert W. Holt, Fadi El-Ghussein, Qun Zhu, Hamid Dehghani, Frederic Leblond, and Brian W. Pogue  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 11, pp. 3021-3036 (2011)
http://dx.doi.org/10.1364/BOE.2.003021


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Abstract

In this study, several key optimization steps are outlined for a non-contact, time-correlated single photon counting small animal optical tomography system, using simultaneous collection of both fluorescence and transmittance data. The system is presented for time-domain image reconstruction in vivo, illustrating the sensitivity from single photon counting and the calibration steps needed to accurately process the data. In particular, laser time- and amplitude-referencing, detector and filter calibrations, and collection of a suitable instrument response function are all presented in the context of time-domain fluorescence tomography and a fully automated workflow is described. Preliminary phantom time-domain reconstructed images demonstrate the fidelity of the workflow for fluorescence tomography based on signal from multiple time gates.

© 2011 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3660) Medical optics and biotechnology : Light propagation in tissues

ToC Category:
Diffuse Optical Imaging

History
Original Manuscript: July 11, 2011
Revised Manuscript: August 17, 2011
Manuscript Accepted: September 22, 2011
Published: October 5, 2011

Virtual Issues
Advances in Optics for Biotechnology, Medicine, and Surgery (2011) Biomedical Optics Express

Citation
Kenneth M. Tichauer, Robert W. Holt, Fadi El-Ghussein, Qun Zhu, Hamid Dehghani, Frederic Leblond, and Brian W. Pogue, "Imaging workflow and calibration for CT-guided time-domain fluorescence tomography," Biomed. Opt. Express 2, 3021-3036 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-11-3021


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