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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 11 — Nov. 1, 2011
  • pp: 3094–3108

Novel non-contact retina camera for the rat and its application to dynamic retinal vessel analysis

Dietmar Link, Clemens Strohmaier, Bernd U. Seifert, Thomas Riemer, Herbert A. Reitsamer, Jens Haueisen, and Walthard Vilser  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 11, pp. 3094-3108 (2011)
http://dx.doi.org/10.1364/BOE.2.003094


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Abstract

We present a novel non-invasive and non-contact system for reflex-free retinal imaging and dynamic retinal vessel analysis in the rat. Theoretical analysis was performed prior to development of the new optical design, taking into account the optical properties of the rat eye and its specific illumination and imaging requirements. A novel optical model of the rat eye was developed for use with standard optical design software, facilitating both sequential and non-sequential modes. A retinal camera for the rat was constructed using standard optical and mechanical components. The addition of a customized illumination unit and existing standard software enabled dynamic vessel analysis. Seven-minute in-vivo vessel diameter recordings performed on 9 Brown-Norway rats showed stable readings. On average, the coefficient of variation was (1.1 ± 0.19) % for the arteries and (0.6 ± 0.08) % for the veins. The slope of the linear regression analysis was (0.56 ± 0.26) % for the arteries and (0.15 ± 0.27) % for the veins. In conclusion, the device can be used in basic studies of retinal vessel behavior.

© 2011 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(330.4460) Vision, color, and visual optics : Ophthalmic optics and devices
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging
(170.2945) Medical optics and biotechnology : Illumination design
(330.7325) Vision, color, and visual optics : Visual optics, metrology

ToC Category:
Ophthalmology Applications

History
Original Manuscript: July 18, 2011
Revised Manuscript: October 1, 2011
Manuscript Accepted: October 6, 2011
Published: October 17, 2011

Citation
Dietmar Link, Clemens Strohmaier, Bernd U. Seifert, Thomas Riemer, Herbert A. Reitsamer, Jens Haueisen, and Walthard Vilser, "Novel non-contact retina camera for the rat and its application to dynamic retinal vessel analysis," Biomed. Opt. Express 2, 3094-3108 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-11-3094


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