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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 11 — Nov. 1, 2011
  • pp: 3223–3231

Light assisted antibody immobilization for bio-sensing

Bartolomeo Della Ventura, Luigi Schiavo, Carlo Altucci, Rosario Esposito, and Raffaele Velotta  »View Author Affiliations

Biomedical Optics Express, Vol. 2, Issue 11, pp. 3223-3231 (2011)

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Ultrashort UV pulses at 258 nm with repetition rate of 10 kHz have been used to irradiate buffer solution of antibody. The tryptophan residues strongly absorb this radiation thus becoming capable to disrupt the disulfide bridges located next to them. Due to their high reactivity the opened bridges can anchor a gold plate more efficiently than other sites of the macromolecule giving rise to preferential orientations of the variable part of the antibody. UV irradiation has been applied to anchor antiIgG antibody to the electrode of a Quartz Crystal Microbalance (QCM) that lends itself as a sensor, the antibody acting as the bio-receptor. An increase of the QCM sensitivity and of the linear range has been measured when the antibody is irradiated with UV laser pulses. The photo-induced reactions leading to disulfide bridge breakage have been analyzed by means of a chemical assay that confirms our explanation. The control of disulfide bridges by UV light paves the way to important applications for sensing purpose since cysteine in combination with tryptophan can act as a hook to link refractory bio-receptors to surfaces.

© 2011 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(350.3450) Other areas of optics : Laser-induced chemistry
(280.1415) Remote sensing and sensors : Biological sensing and sensors

ToC Category:
Biosensors and Molecular Diagnostics

Original Manuscript: September 14, 2011
Revised Manuscript: October 17, 2011
Manuscript Accepted: October 17, 2011
Published: October 28, 2011

Bartolomeo Della Ventura, Luigi Schiavo, Carlo Altucci, Rosario Esposito, and Raffaele Velotta, "Light assisted antibody immobilization for bio-sensing," Biomed. Opt. Express 2, 3223-3231 (2011)

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