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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 11 — Nov. 1, 2011
  • pp: 3223–3231

Light assisted antibody immobilization for bio-sensing

Bartolomeo Della Ventura, Luigi Schiavo, Carlo Altucci, Rosario Esposito, and Raffaele Velotta  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 11, pp. 3223-3231 (2011)
http://dx.doi.org/10.1364/BOE.2.003223


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Abstract

Ultrashort UV pulses at 258 nm with repetition rate of 10 kHz have been used to irradiate buffer solution of antibody. The tryptophan residues strongly absorb this radiation thus becoming capable to disrupt the disulfide bridges located next to them. Due to their high reactivity the opened bridges can anchor a gold plate more efficiently than other sites of the macromolecule giving rise to preferential orientations of the variable part of the antibody. UV irradiation has been applied to anchor antiIgG antibody to the electrode of a Quartz Crystal Microbalance (QCM) that lends itself as a sensor, the antibody acting as the bio-receptor. An increase of the QCM sensitivity and of the linear range has been measured when the antibody is irradiated with UV laser pulses. The photo-induced reactions leading to disulfide bridge breakage have been analyzed by means of a chemical assay that confirms our explanation. The control of disulfide bridges by UV light paves the way to important applications for sensing purpose since cysteine in combination with tryptophan can act as a hook to link refractory bio-receptors to surfaces.

© 2011 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(350.3450) Other areas of optics : Laser-induced chemistry
(280.1415) Remote sensing and sensors : Biological sensing and sensors

ToC Category:
Biosensors and Molecular Diagnostics

History
Original Manuscript: September 14, 2011
Revised Manuscript: October 17, 2011
Manuscript Accepted: October 17, 2011
Published: October 28, 2011

Citation
Bartolomeo Della Ventura, Luigi Schiavo, Carlo Altucci, Rosario Esposito, and Raffaele Velotta, "Light assisted antibody immobilization for bio-sensing," Biomed. Opt. Express 2, 3223-3231 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-11-3223


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References

  1. M. A. Cooper and V. T. Singleton, “A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions,” J. Mol. Recognit.20(3), 154–184 (2007). [CrossRef] [PubMed]
  2. J. L. Arlett, E. B. Myers, and M. L. Roukes, “Comparative advantages of mechanical biosensors,” Nat. Nanotechnol.6(4), 203–215 (2011). [CrossRef] [PubMed]
  3. Y. G. Lee and K. S. Chang, “Application of a flow type quartz crystal microbalance immunosensor for real time determination of cattle bovine ephemeral fever virus in liquid,” Talanta65(5), 1335–1342 (2005). [CrossRef] [PubMed]
  4. X. Su, Y. J. Wu, and W. Knoll, “Comparison of surface plasmon resonance spectroscopy and quartz crystal microbalance techniques for studying DNA assembly and hybridization,” Biosens. Bioelectron.21(5), 719–726 (2005). [CrossRef] [PubMed]
  5. E. Ostuni, L. Yan, and G. M. Whitesides, “The interaction of proteins with self-assembled monolayers of alkanethiolates on gold and silver,” Colloids Surf.13, 3–30 (1999).
  6. Y. Ruan, T. C. Foo, S. Warren-Smith, P. Hoffmann, R. C. Moore, H. Ebendorff-Heidepriem, and T. M. Monro, “Antibody immobilization within glass microstructured fibers: a route to sensitive and selective biosensors,” Opt. Express16(22), 18514–18523 (2008). [CrossRef] [PubMed]
  7. J. R. Ott, M. Heuck, C. Agger, P. D. Rasmussen, and O. Bang, “Label-free and selective nonlinear fiber-optical biosensing,” Opt. Express16(25), 20834–20847 (2008). [CrossRef] [PubMed]
  8. H. N. Daghestani and B. W. Day, “Theory and applications of surface plasmon resonance, resonant mirror, resonant waveguide grating, and dual polarization interferometry biosensors,” Sensors (Basel Switzerland)10(11), 9630–9646 (2010). [CrossRef]
  9. M. T. Neves-Petersen, T. Snabe, S. Klitgaard, M. Duroux, and S. B. Petersen, “Photonic activation of disulfide bridges achieves oriented protein immobilization on biosensor surfaces,” Protein Sci.15(2), 343–351 (2006). [CrossRef] [PubMed]
  10. C. A. Janeway, Jr., P. Travers, M. Walport, and M. J. Shlomchiket, Immunobiology (Garland Science, 2001).
  11. M. T. Neves-Petersen, Z. Gryczynski, J. Lakowicz, P. Fojan, S. Pedersen, E. Petersen, and S. Bjørn Petersen, “High probability of disrupting a disulphide bridge mediated by an endogenous excited tryptophan residue,” Protein Sci.11(3), 588–600 (2002). [CrossRef] [PubMed]
  12. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Springer, 2006).
  13. R. Weinkauf, P. Aicher, G. Wesley, J. Grotemeyer, and E. W. Schlag, “Femtosecond versus nanosecond multiphoton ionization and dissociation of large molecules,” J. Phys. Chem.98(34), 8381–8391 (1994). [CrossRef]
  14. B. Gu, K. Lou, H. T. Wang, and W. Ji, “Dynamics of two-photon-induced three-photon absorption in nanosecond, picosecond, and femtosecond regimes,” Opt. Lett.35(3), 417–419 (2010). [CrossRef] [PubMed]
  15. DataBank RCSB PDB, IgG structure ( http://www.rcsb.org/pdb/explore/explore.do?structureId=3I75 ).
  16. T. R. Ioerger, C. Du, and D. S. Linthicum, “Conservation of cys-cys trp structural triads and their geometry in the protein domains of immunoglobulin superfamily members,” Mol. Immunol.36(6), 373–386 (1999). [CrossRef] [PubMed]
  17. G. Z. Sauerbrey, “Verwendung von Schwingquarzen zur Wägung dünner Schichten und zur Mikrowägung,” Z. Phys.155(2), 206–222 (1959). [CrossRef]
  18. G. L. Ellman, “Tissue sulfhydryl groups,” Arch. Biochem. Biophys.82(1), 70–77 (1959). [CrossRef] [PubMed]
  19. C. Yao, T. Zhu, Y. Qi, Y. Zhao, H. Xia, and W. Fu, “Development of a quartz crystal microbalance biosensor with aptamer as bio-recognition element,” Sensors (Basel Switzerland)10(6), 5859–5871 (2010). [CrossRef]

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