OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 12 — Dec. 1, 2011
  • pp: 3259–3266

Optics InfoBase > Biomedical Optics Express > Volume 2 > Issue 12 > Page 3259

Blood testing at the single cell level using quantitative phase and amplitude microscopy

Mustafa Mir, Krishnarao Tangella, and Gabriel Popescu  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 12, pp. 3259-3266 (2011)
http://dx.doi.org/10.1364/BOE.2.003259


View Full Text Article

Enhanced HTML    Acrobat PDF (1084 KB)


Advanced Search

Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

It has recently been shown that quantitative phase imaging methods can provide clinically relevant parameters for red blood cell analysis with unprecedented detail and sensitivity. Since the quantitative phase information is dependent on both the thickness and refractive index, a major limitation to clinical translation has been a simple and practical approach to measure both simultaneously. Here we demonstrate both theoretically and experimentally that, by combining quantitative phase with a single absorption measurement, it is possible to measure both quantities at the single cell level. We validate this approach by comparing our results to those acquired using a clinical blood analyzer. This approach to decouple the thickness and refractive index for red blood cells may be used with any quantitative phase imaging method that can operate in tandem with bright field microscopy at the Soret-band wavelength.

© 2011 OSA

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1610) Medical optics and biotechnology : Clinical applications
(180.3170) Microscopy : Interference microscopy
(300.1030) Spectroscopy : Absorption

ToC Category:
Microscopy

History
Original Manuscript: September 27, 2011
Revised Manuscript: October 21, 2011
Manuscript Accepted: November 6, 2011
Published: November 7, 2011

Citation
Mustafa Mir, Krishnarao Tangella, and Gabriel Popescu, "Blood testing at the single cell level using quantitative phase and amplitude microscopy," Biomed. Opt. Express 2, 3259-3266 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-12-3259


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. B. J. Bain, Blood Cells A Practical Guide, 3rd ed. (Blackwell Science, London, 2002).
  2. B. J. Bain, “Diagnosis from the blood smear,” N. Engl. J. Med.353(5), 498–507 (2005). [CrossRef] [PubMed]
  3. H. H. Billett, M. E. Fabry, and R. L. Nagel, “Hemoglobin distribution width: a rapid assessment of dense red cells in the steady state and during painful crisis in sickle cell anemia,” J. Lab. Clin. Med.112(3), 339–344 (1988). [PubMed]
  4. M. Piagnerelli, K. Zouaoui Boudjeltia, D. Brohee, A. Vereerstraeten, P. Piro, J.-L. Vincent, and M. Vanhaeverbeek, “Assessment of erythrocyte shape by flow cytometry techniques,” J. Clin. Pathol.60(5), 549–554 (2007). [CrossRef] [PubMed]
  5. G. Rusciano, “Experimental analysis of Hb oxy-deoxy transition in single optically stretched red blood cells,” Phys. Med.26(4), 233–239 (2010). [CrossRef] [PubMed]
  6. S. Winoto-Morbach, W. Müller-Ruchholtz, and V. Tchikov, “Magnetophoresis: II. Quantification of iron and hemoglobin content at the single erythrocyte level,” J. Clin. Lab. Anal.9(1), 42–46 (1995). [CrossRef] [PubMed]
  7. A. Esposito, T. Tiffert, J. M. Mauritz, S. Schlachter, L. H. Bannister, C. F. Kaminski, and V. L. Lew, “FRET imaging of hemoglobin concentration in Plasmodium falciparum-infected red cells,” PLoS ONE3(11), e3780 (2008). [CrossRef] [PubMed]
  8. K. A. Sem’yanov, P. A. Tarasov, J. T. Soini, A. K. Petrov, and V. P. Maltsev, “Calibration-free method to determine the size and hemoglobin concentration of individual red blood cells from light scattering,” Appl. Opt.39(31), 5884–5889 (2000). [CrossRef] [PubMed]
  9. M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011). [CrossRef] [PubMed]
  10. M. Mir, Z. Wang, K. Tangella, and G. Popescu, “Diffraction phase cytometry: blood on a CD-ROM,” Opt. Express17(4), 2579–2585 (2009). [CrossRef] [PubMed]
  11. Y. Park, C. A. Best, T. Auth, N. S. Gov, S. A. Safran, G. Popescu, S. Suresh, and M. S. Feld, “Metabolic remodeling of the human red blood cell membrane,” Proc. Natl. Acad. Sci. U.S.A.107(4), 1289–1294 (2010). [CrossRef] [PubMed]
  12. Y. Park, C. A. Best, K. Badizadegan, R. R. Dasari, M. S. Feld, T. Kuriabova, M. L. Henle, A. J. Levine, and G. Popescu, “Measurement of red blood cell mechanics during morphological changes,” Proc. Natl. Acad. Sci. U.S.A.107(15), 6731–6736 (2010). [CrossRef] [PubMed]
  13. Y. Park, M. Diez-Silva, D. Fu, G. Popescu, W. Choi, I. Barman, S. Suresh, and M. S. Feld, “Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells,” J. Biomed. Opt.15(2), 020506 (2010). [CrossRef] [PubMed]
  14. G. Popescu, Y. Park, R. R. Dasari, K. Badizadegan, and M. S. Feld, “Coherence properties of red blood cell membrane motions,” Phys. Rev. E Stat. Nonlin. Soft Matter Phys.76(3), 031902 (2007). [CrossRef] [PubMed]
  15. G. Popescu, Y. Park, N. Lue, C. Best-Popescu, L. Deflores, R. R. Dasari, M. S. Feld, and K. Badizadegan, “Optical imaging of cell mass and growth dynamics,” Am. J. Physiol. Cell Physiol.295(2), C538–C544 (2008). [CrossRef] [PubMed]
  16. G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Observation of dynamic subdomains in red blood cells,” J. Biomed. Opt.11(4), 040503 (2006). [CrossRef] [PubMed]
  17. S. Seo, S. O. Isikman, I. Sencan, O. Mudanyali, T. W. Su, W. Bishara, A. Erlinger, and A. Ozcan, “High-throughput lens-free blood analysis on a chip,” Anal. Chem.82(11), 4621–4627 (2010). [CrossRef] [PubMed]
  18. S. Seo, T. W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, “Lensfree holographic imaging for on-chip cytometry and diagnostics,” Lab Chip9(6), 777–787 (2009). [CrossRef] [PubMed]
  19. N. T. Shaked, L. L. Satterwhite, M. J. Telen, G. A. Truskey, and A. Wax, “Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry,” J. Biomed. Opt.16(3), 030506 (2011). [CrossRef] [PubMed]
  20. A. R. Brazhe, A. I. Yusipovich, E. Y. Parshina, N. Y. Brysgalova, N. A. Brazhe, A. G. Lomakin, G. G. Levin, and G. V. Maksimov, “Laser interference microscopy in erythrocyte study,” J. Appl. Phys.105, 102037(2009).
  21. M. Mir, H. Ding, Z. Wang, J. Reedy, K. Tangella, and G. Popescu, “Blood screening using diffraction phase cytometry,” J. Biomed. Opt.15(2), 027016 (2010). [CrossRef] [PubMed]
  22. B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer,” Cytometry A73A(10), 895–903 (2008). [CrossRef] [PubMed]
  23. B. Rappaz, F. Charrière, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium,” Opt. Lett.33(7), 744–746 (2008). [CrossRef] [PubMed]
  24. B. Rappaz, P. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. Magistretti, “Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy,” Opt. Express13(23), 9361–9373 (2005). [CrossRef] [PubMed]
  25. Y. K. Park, T. Yamauchi, W. Choi, R. Dasari, and M. S. Feld, “Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells,” Opt. Lett.34(23), 3668–3670 (2009). [CrossRef] [PubMed]
  26. Z. Wang, L. Millet, M. Mir, H. Ding, S. Unarunotai, J. Rogers, M. U. Gillette, and G. Popescu, “Spatial light interference microscopy (SLIM),” Opt. Express19(2), 1016–1026 (2011). [CrossRef] [PubMed]
  27. Z. Wang and G. Popescu, “Quantitative phase imaging with broadband fields,” Appl. Phys. Lett.96(5), 051117 (2010). [CrossRef]
  28. R. Barer, “Refractometry and interferometry of living cells,” J. Opt. Soc. Am.47(6), 545–556 (1957). [CrossRef] [PubMed]
  29. R. Barer, J. B. Howie, K. F. Ross, and S. Tkaczyk, “Applications of refractometry in haematology,” J. Physiol.120(4), 67P–68P (1953). [PubMed]
  30. R. Barer and K. A. Ross, “Refractometry of living cells,” J. Physiol.118(2), 38P–39P (1952). [PubMed]
  31. R. Barer and S. Tkaczyk, “Refractive index of concentrated protein solutions,” Nature173(4409), 821–822 (1954). [CrossRef] [PubMed]
  32. Z. Wang, I. S. Chun, X. Li, Z. Y. Ong, E. Pop, L. Millet, M. U. Gillette, and G. Popescu, “Topography and refractometry of nanostructures using spatial light interference microscopy,” Opt. Lett.35(2), 208–210 (2010). [CrossRef] [PubMed]
  33. S. Prahl, “Tabulated molar extinction coefficient for hemoglobin in water” (1998), http://omlc.ogi.edu/spectra/hemoglobin/summary.html .

Cited By

 Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited