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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 2, Iss. 12 — Dec. 1, 2011
  • pp: 3321–3333

Characterization of optical properties of ZnO nanoparticles for quantitative imaging of transdermal transport

Zhen Song, Timothy A. Kelf, Washington H. Sanchez, Michael S. Roberts, Jaro Rička, Martin Frenz, and Andrei V. Zvyagin  »View Author Affiliations


Biomedical Optics Express, Vol. 2, Issue 12, pp. 3321-3333 (2011)
http://dx.doi.org/10.1364/BOE.2.003321


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Abstract

Widespread applications of ZnO nanoparticles (NP) in sun-blocking cosmetic products have raised safety concerns related to their potential transdermal penetration and resultant cytotoxicity. Nonlinear optical microscopy provides means for high-contrast imaging of ZnO NPs lending in vitro and in vivo assessment of the nanoparticle uptake in skin, provided their nonlinear optical properties are characterized. We report on this characterization using ZnO NP commercial product, Zinclear, mean-sized 21 nm. Two-photon action cross-section of this bandgap material (Ebg = 3.37 eV, λbg = 370 nm) measured by two techniques yielded consistent results of η ZnO σ ZnO ( 2ph ) = 6.2 ± 0.8 μGM at 795 nm, and 32 ± 6 μGM at 770 nm per unit ZnO crystal cell, with the quantum efficiency of η ZnO = (0.9 ± 0.2) %. In order to demonstrate the quantitative imaging, nonlinear optical microscopy images of the excised human skin topically treated with Zinclear were acquired and processed using σ ZnO ( 2ph ) and η ZnO values yielding nanoparticle concentration map in skin. Accumulations of Zinclear ZnO nanoparticles were detected only on the skin surface and in skin folds reaching concentrations of 800 NPs per μm3.

© 2011 OSA

OCIS Codes
(160.2540) Materials : Fluorescent and luminescent materials
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(250.5230) Optoelectronics : Photoluminescence
(160.4236) Materials : Nanomaterials
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Dermatological Applications

History
Original Manuscript: October 7, 2011
Revised Manuscript: November 6, 2011
Manuscript Accepted: November 8, 2011
Published: November 15, 2011

Citation
Zhen Song, Timothy A. Kelf, Washington H. Sanchez, Michael S. Roberts, Jaro Rička, Martin Frenz, and Andrei V. Zvyagin, "Characterization of optical properties of ZnO nanoparticles for quantitative imaging of transdermal transport," Biomed. Opt. Express 2, 3321-3333 (2011)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-2-12-3321


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